Rombel I T, Lamont I L
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
J Gen Microbiol. 1992 Jan;138(1):181-7. doi: 10.1099/00221287-138-1-181.
Many species of pseudomonads produce fluorescent siderophores involved in iron uptake. We have investigated the DNA homology between the siderophore synthesis genes of an opportunist animal pathogen, Pseudomonas aeruginosa, and three plant-associated species Pseudomonas syringae, Pseudomonas putida and Pseudomonas sp. B10. There is extensive homology between the DNA from the different species, consistent with the suggestion that the different siderophore synthesis genes have evolved from the same ancestral set of genes. The existence of DNA homology allowed us to clone some of the siderophore synthesis genes from P. aeruginosa, and genetic mapping indicates that the cloned DNA lies in a locus previously identified as being involved in siderophore production.
许多假单胞菌属的物种会产生参与铁摄取的荧光铁载体。我们研究了一种机会性动物病原体铜绿假单胞菌与三种植物相关物种丁香假单胞菌、恶臭假单胞菌和假单胞菌属B10的铁载体合成基因之间的DNA同源性。不同物种的DNA之间存在广泛的同源性，这与不同的铁载体合成基因是从同一祖先基因集进化而来的观点一致。DNA同源性的存在使我们能够从铜绿假单胞菌中克隆一些铁载体合成基因，遗传图谱表明克隆的DNA位于先前确定参与铁载体产生的一个位点上。
