McMorran B J, Merriman M E, Rombel I T, Lamont I L
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Gene. 1996 Oct 17;176(1-2):55-9. doi: 10.1016/0378-1119(96)00209-0.
Pseudomonas aeruginosa (Pa) strain PAO synthesises a siderophore, pyoverdine (Pvd), when grown under conditions of iron starvation. Pvd consists of a dihydroxyquinoline group attached to an 8-amino-acid-residue peptide. DNA spanning at least 78 kb of the chromosome is required for Pvd synthesis, but to date only three genes involved in this process have been characterised. We report the characterisation of a fourth Pa gene, pvdE, which we show to be required for Pvd synthesis. The deduced amino acid sequence of PvdE indicates that the protein is a member of the ATP-binding-cassette (ABC) family of membrane transporter proteins, and this is the first example of the involvement of an ABC-type protein in siderophore synthesis.
铜绿假单胞菌（Pa）菌株PAO在铁饥饿条件下生长时会合成一种铁载体——绿脓菌素（Pvd）。Pvd由一个连接到一个8氨基酸残基肽上的二羟基喹啉基团组成。Pvd合成需要跨越至少78 kb染色体的DNA，但迄今为止，参与这一过程的基因只有三个已被鉴定。我们报告了第四个Pa基因pvdE的鉴定，我们发现它是Pvd合成所必需的。PvdE推导的氨基酸序列表明该蛋白质是膜转运蛋白的ATP结合盒（ABC）家族的成员，这是ABC型蛋白参与铁载体合成的首个例子。
