Tipnis U R, Boor P J
Department of Pathology, University of Texas Medical Branch, Galveston 77550.
Regul Pept. 1992 Feb 18;37(3):227-36. doi: 10.1016/0167-0115(92)90617-4.
The aim of the present study was to evaluate in vivo the role of polyamines in the secretion of atrial natriuretic peptide (ANP). alpha-Difluoromethylornithine (DFMO) which inhibits ornithine decarboxylase activity and polyamine synthesis was given in drinking water and through intraperitoneal administration to Sprague-Dawley rats. Carotid artery was cannulated for collection of blood samples and measurement of blood pressure following the administration of arginine-vasopressin (AVP). Analysis of polyamines in cardiac tissue indicated that DFMO treatment decreased contents of putrescine and spermidine in cardiac tissue by 80% and 48%, respectively. Quantitation of ANP in plasma by radioimmunoassay indicated that both basal and stimulated levels of ANP in DFMO-treated animals were 21.5% and 50% of those in control rats. The administration of putrescine restored the levels of basal and AVP-stimulated levels of ANP in plasma which confirmed that DFMO effect on ANP secretion occurred specifically through the polyamine pathway.
本研究的目的是在体内评估多胺在心房利钠肽（ANP）分泌中的作用。将抑制鸟氨酸脱羧酶活性和多胺合成的α-二氟甲基鸟氨酸（DFMO）通过饮用水和腹腔注射给予Sprague-Dawley大鼠。在给予精氨酸加压素（AVP）后，插入颈动脉以采集血样并测量血压。心脏组织中多胺的分析表明，DFMO处理使心脏组织中腐胺和亚精胺的含量分别降低了80%和48%。通过放射免疫测定法对血浆中的ANP进行定量分析表明，DFMO处理动物的ANP基础水平和刺激水平分别为对照大鼠的21.5%和50%。腐胺的给药恢复了血浆中ANP的基础水平和AVP刺激水平，这证实了DFMO对ANP分泌的影响是通过多胺途径特异性发生的。
