Westphal J R, Tax W J, Willems H W, Koene R A, Ruiter D J, De Waal R M
Institute of Pathology, University Hospital Nijmegen, The Netherlands.
Scand J Immunol. 1992 Apr;35(4):449-57. doi: 10.1111/j.1365-3083.1992.tb02880.x.
Monoclonal antibodies to CD3 can induce proliferation of resting T cells. In vitro this effect is dependent on the presence of monocytes. They serve as accessory cells providing a co-stimulatory signal after cross-linking of the antibody-coated TcR/CD3 complex by the Fc receptor on the monocytes. We have studied whether endothelial cells can replace monocytes with regard to this function. Highly purified T-cell preparations were cultured in the presence of anti-CD3 antibody, purified monocytes, and human umbilical vein endothelial cells. Anti-CD3 and endothelial cells alone were unable to support T-cell proliferation, due to lack of FcR expression. Addition, however, of as few as 1000 FcR+ monocytes (0.8% of the number of T cells present) to a coculture of T cells and endothelial cells (EC) in the presence of soluble anti-CD3 resulted in a strong proliferation of T cells. When anti-CD3 was presented in an immobilized form (coated to the culture well or to Sepharose beads), or when phytohaemagglutinin was added to the culture as a cross-linking agent, EC could support T-cell proliferation in the absence of any monocytes. We conclude that EC by themselves cannot support the proliferation of pure T cells induced by soluble anti-CD3, but are potent generators of the co-stimulatory signal(s). They provide a suitable starting material to further define this co-stimulatory activity.
抗CD3单克隆抗体可诱导静息T细胞增殖。在体外，这种效应依赖于单核细胞的存在。它们作为辅助细胞，在单核细胞上的Fc受体使抗体包被的TcR/CD3复合物交联后提供共刺激信号。我们研究了内皮细胞在该功能方面是否能替代单核细胞。将高度纯化的T细胞制剂与抗CD3抗体、纯化的单核细胞和人脐静脉内皮细胞一起培养。单独的抗CD3和内皮细胞由于缺乏FcR表达而无法支持T细胞增殖。然而，在可溶性抗CD3存在的情况下，向T细胞和内皮细胞（EC）的共培养物中加入低至1000个FcR+单核细胞（占存在的T细胞数量的0.8%），会导致T细胞强烈增殖。当抗CD3以固定形式呈现（包被在培养孔或琼脂糖珠上），或者当向培养物中加入植物血凝素作为交联剂时，内皮细胞可以在没有任何单核细胞的情况下支持T细胞增殖。我们得出结论，内皮细胞自身不能支持可溶性抗CD3诱导的纯T细胞增殖，但它们是共刺激信号的有效产生者。它们为进一步确定这种共刺激活性提供了合适的起始材料。
