Ouellette G B, Baayen R P, Chamberland H, Simard M, Rioux D, Charest P M
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 3800, Sainte-Foy, Quebec G1V 4C7, Canada.
Microsc Microanal. 2004 Aug;10(4):449-61. doi: 10.1017/S1431927604040796.
Antibodies to detect pectin in present investigations attached to distinct fibrils in vessel lumina. In carnation infected with an isolate of Fusarium oxysporum f.sp., labeling of pathogen cells also occurred; in a resistant cultivar (cv.), it was coincident with proximate pectin fibrils and linked to altered fungal walls, which was the opposite in the susceptible cv., indicating that hindrance of pathogen ability to degrade pectin may be related to resistance. Labeling of the fungus in culture was nil, except in media containing pectin, showing that pectin is not native to the pathogen. Labeling of fungal walls for cellulose in elm (inoculated with Ophiostoma novo-ulmi) and carnation also occurred, linked to adsorbed host wall components. The chitin probe often attached to dispersed matter, in vessel lumina, traceable to irregularly labeled fungal cells and host wall degradation products. With an anti-horseradish peroxidase probe, host and fungal walls were equally labeled, and with a glucosidase, differences of labeling between these walls were observed, depending on pH of the test solution. Fungal extracellular matter and filamentous structures, present in fungal walls, predominantly in another elm isolate (Phaeotheca dimorphospora), did not label with any of the probes used. However, in cultures of this fungus, extracellular material labeled, even at a distance from the colony margin, with an anti-fimbriae probe.
在当前研究中，用于检测果胶的抗体附着在血管腔中的不同原纤维上。在感染尖孢镰刀菌一个分离株的香石竹中，病原菌细胞也出现了标记；在一个抗病品种中，它与紧邻的果胶原纤维一致，并与真菌细胞壁的改变有关，而在感病品种中情况则相反，这表明病原菌降解果胶能力的受阻可能与抗性有关。在培养物中，除了含有果胶的培养基外，真菌均未被标记，这表明果胶并非病原菌所固有。在榆木（接种新榆长喙壳菌）和香石竹中，真菌细胞壁上也出现了纤维素标记，这与吸附的寄主细胞壁成分有关。几丁质探针常常附着在血管腔中的分散物质上，这些物质可追溯到标记不规则的真菌细胞和寄主细胞壁降解产物。使用抗辣根过氧化物酶探针时，寄主和真菌细胞壁被同等标记；而使用葡糖苷酶时，根据测试溶液的pH值，可观察到这些细胞壁之间标记的差异。在真菌细胞壁中存在的真菌细胞外物质和丝状结构，主要存在于另一种榆木分离株（双型暗隔孢）中，未被所用的任何探针标记。然而，在这种真菌的培养物中，即使在离菌落边缘一定距离处，细胞外物质也能用抗菌毛探针标记。
