Hopfgartner Gérard, Varesio Emmanuel, Tschäppät Viviane, Grivet Chantal, Bourgogne Emmanuel, Leuthold Luc Alexis
Laboratory of Pharmaceutical Analytical Chemistry, Life Sciences Mass Spectrometry, University of Geneva, 20 Bd. d'Yvoy, 1211 4, Switzerland.
J Mass Spectrom. 2004 Aug;39(8):845-55. doi: 10.1002/jms.659.
Recently, linear ion traps (LITs) have been combined with quadrupole (Q), time-of-flight (TOF) and Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS). LITs can be used either as ion accumulation devices or as commercially available, stand-alone mass spectrometers with MSn capabilities. The combination of triple quadrupole MS with LIT technology in the form of an instrument of configuration QqLIT, using axial ejection, is particularly interesting, because this instrument retains the classical triple quadrupole scan functions such as selected reaction monitoring (SRM), product ion (PI), neutral loss (NL) and precursor ion (PC) while also providing access to sensitive ion trap experiments. For small molecules, quantitative and qualitative analysis can be performed using the same instrument. In addition, for peptide analysis, the enhanced multiply charged (EMC) scan allows an increase in selectivity, while the time-delayed fragmentation (TDF) scan provides additional structural information. Various methods of operating the hybrid instrument are described for the case of the commercial Q TRAP (AB/MDS Sciex) and applications to drug metabolism analysis, quantitative confirmatory analysis, peptides analysis and automated nanoelectrospray (ESI-chip-MS) analysis are discussed.
最近，线性离子阱（LIT）已与四极杆（Q）、飞行时间（TOF）和傅里叶变换离子回旋共振（FT-ICR）质谱（MS）相结合。LIT既可以用作离子累积装置，也可以用作具备MSn功能的市售独立质谱仪。以QqLIT配置的仪器形式将三重四极杆质谱与LIT技术相结合，并采用轴向喷射，这一点特别引人关注，因为该仪器保留了经典的三重四极杆扫描功能，如选择反应监测（SRM）、产物离子（PI）、中性丢失（NL）和前体离子（PC），同时还能进行灵敏的离子阱实验。对于小分子，可以使用同一台仪器进行定量和定性分析。此外，对于肽分析，增强型多电荷（EMC）扫描可提高选择性，而延时碎裂（TDF）扫描可提供额外的结构信息。针对商用Q TRAP（AB/MDS Sciex）的情况，描述了混合仪器的各种操作方法，并讨论了其在药物代谢分析、定量确证分析、肽分析和自动纳喷（ESI芯片-MS）分析中的应用。
