Raczkowska Adrianna, Brzostek Katarzyna
Institute of Microbiology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Pol J Microbiol. 2004;53(1):11-6.
Yersinia enterocolitica is a human pathogen that causes gastroenteric infections. Various environmental signals control the expression of the virulence factors in pathogenic Y. enterocolitica strains. OmpR, a global transcriptional regulator controls the expression of a wide spectrum of genes, some of which are required for virulence. In this study, we amplified, cloned and sequenced a Y. enterocolitica Ye9 ompR gene. Deduced amino acid sequence has been shown to have 98% homology to the Y. enterocolitica O:8, Y. pestis, S. typhi and S. enterica serovar Typhimurium OmpR proteins. Additional cell culture experiments was performed to investigate whether OmpR takes part in the virulence of Y. enterocolitica. We found that the Y. enterocolitica ompR mutant was unable to invade HeLa cells. In conclusion, we have shown that OmpR is a very highly conserved protein among enteric bacterial pathogens which plays an important role in the Y. enterocolitica virulence.
小肠结肠炎耶尔森菌是一种可引发胃肠感染的人类病原体。多种环境信号控制着致病性小肠结肠炎耶尔森菌菌株中毒力因子的表达。OmpR是一种全局转录调节因子，可控制多种基因的表达，其中一些基因是毒力所必需的。在本研究中，我们对小肠结肠炎耶尔森菌Ye9的ompR基因进行了扩增、克隆和测序。推导的氨基酸序列已显示与小肠结肠炎耶尔森菌O:8、鼠疫耶尔森菌、伤寒沙门菌和鼠伤寒沙门菌OmpR蛋白具有98%的同源性。进行了额外的细胞培养实验，以研究OmpR是否参与小肠结肠炎耶尔森菌的毒力。我们发现小肠结肠炎耶尔森菌ompR突变体无法侵袭HeLa细胞。总之，我们已经表明，OmpR是肠道细菌病原体中一种高度保守的蛋白质，在小肠结肠炎耶尔森菌的毒力中起重要作用。
