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四种真菌气溶胶采样仪器的现场比较:采样器间的校准及注意事项

A field comparison of four fungal aerosol sampling instruments: inter-sampler calibrations and caveats.

作者信息

Lee K S, Teschke K, Brauer M, Bartlett K H

机构信息

School of Occupational and Environmental Hygiene, University of British Columbia, Vancouver, BC, Canada.

出版信息

Indoor Air. 2004 Oct;14(5):367-72. doi: 10.1111/j.1600-0668.2004.00260.x.

DOI:10.1111/j.1600-0668.2004.00260.x
PMID:15330797
Abstract

UNLABELLED

Four bioaerosol samplers (Reuter Centrifugal, Andersen N6 Single Stage, Surface Air System Super 90, and Air-o-Cell) were used to take c. 300 side-by-side measurements at 75 public building sites. Regression models were developed to examine the relationships between each method pair. The models demonstrate that measurements from these instruments are not directly comparable, requiring inter-instrument calibration. Sampling location (indoor vs. outdoor) was a confounder in all the pairwise comparisons between samplers. In addition, the slopes of the relationships between all method pairs except one differed in indoor vs. outdoor locations. These results emphasize that direct comparisons between methods should not be undergone without prior calibration. Where measurement circumstances are similar to those of this study, the regression models might serve as a basis to convert measurements made with one instrument to those made with another. However, the robustness and generalizability of the models in different measurement settings needs to be assessed.

PRACTICAL IMPLICATIONS

Many different bioaerosol sampling devices are in common use for indoor air quality studies. If data from research studies are to be compared, an approximation of the relationships between the equipment would be useful. A comparison of three culturable sampling devices (Andersen N6, SAS 90, RCS) and one particulate sampling device (Air-o-Cell) collecting simultaneous samples under field conditions showed high linear correlations between methods. However, while direct comparisons between sampling data were not possible, the regression models reported here explained 60-85% of the variance in fungal concentrations, and underscored the importance of the effect of environment on measurement.

摘要

未标注

使用了四种生物气溶胶采样器(路透社离心式、安德森N6单级、表面空气系统超级90型和空气细胞采样器)在75个公共建筑场所进行了约300次并排测量。建立了回归模型以检验各方法对之间的关系。模型表明,这些仪器的测量结果不能直接比较,需要进行仪器间校准。采样位置(室内与室外)在采样器之间的所有成对比较中都是一个混杂因素。此外,除一组外,所有方法对之间关系的斜率在室内和室外位置有所不同。这些结果强调,未经事先校准不应直接进行方法之间的比较。在测量情况与本研究相似的情况下,回归模型可作为将一种仪器的测量结果转换为另一种仪器测量结果的基础。然而,需要评估模型在不同测量设置中的稳健性和通用性。

实际意义

许多不同的生物气溶胶采样设备常用于室内空气质量研究。如果要比较研究数据,了解设备之间关系的近似值将很有用。在现场条件下对三种可培养采样设备(安德森N6、SAS 90、RCS)和一种颗粒采样设备(空气细胞采样器)同时采集的样本进行比较,结果显示各方法之间具有高度线性相关性。然而,虽然无法直接比较采样数据,但此处报告的回归模型解释了真菌浓度方差的60 - 85%,并强调了环境对测量影响的重要性。

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