Távora L G F, Gambale W, Heins-Vaccari E M, Arriagada G L H, Lacaz C S, Santos C R, Levin A S
Departamento de Moléstias Infecciosas, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
Braz J Med Biol Res. 2003 May;36(5):613-6. doi: 10.1590/s0100-879x2003000500008. Epub 2003 Apr 22.
Many studies have attempted to evaluate the importance of airborne fungi in the development of invasive fungal infection, especially for immunocompromised hosts. Several kinds of instruments are available to quantitate fungal propagule levels in air. We compared the performance of the most frequently used air sampler, the Andersen sampler with six stages, with a portable one, the Reuter centrifugal sampler (RCS). A total of 84 samples were analyzed, 42 with each sampler. Twenty-eight different fungal genera were identified in samples analyzed with the Andersen instrument. In samples obtained with the RCS only seven different fungal genera were identified. The three most frequently isolated genera in samples analyzed with both devices were Penicillium, Aspergillus and Cladophialophora. In areas supplied with a high efficiency particulate air filter, fungal spore levels were usually lower when compared to areas without these filters. There was a significant correlation between total fungal propagule measurements taken with both devices on each sampling occasion (Pearson coefficient = 0.50). However, the Andersen device recovered a broader spectrum of fungi. We conclude that the RCS can be used for quantitative estimates of airborne microbiological concentrations. For qualitative studies, however, this device cannot be recommended.
许多研究试图评估空气传播真菌在侵袭性真菌感染发生发展中的重要性，尤其是对于免疫功能低下的宿主。有几种仪器可用于定量空气中的真菌繁殖体水平。我们比较了最常用的空气采样器——六级安德森采样器与便携式的路透离心采样器（RCS）的性能。共分析了84个样本，每个采样器各42个。在用安德森仪器分析的样本中鉴定出28个不同的真菌属。在用RCS采集的样本中仅鉴定出7个不同的真菌属。两种仪器分析的样本中最常分离出的三个属是青霉属、曲霉属和枝孢瓶霉属。在配备高效空气过滤器的区域，与没有这些过滤器的区域相比，真菌孢子水平通常较低。在每个采样时刻，两种仪器测得的总真菌繁殖体之间存在显著相关性（皮尔逊系数=0.50）。然而，安德森仪器能检测到更广泛的真菌种类。我们得出结论，RCS可用于定量估计空气中微生物的浓度。然而，对于定性研究，不推荐使用该仪器。
