Ory Jeramia J, Griffith Cara L, Doering Tamara L
Washington University School of Medicine, Department of Molecular Microbiology, 660 S Euclid Avenue, Campus Box 8230, Saint Louis, MO 63110, USA.
Yeast. 2004 Aug;21(11):919-26. doi: 10.1002/yea.1139.
Cryptococcus neoformans is an opportunistic fungal pathogen responsible for serious meningitis. Although many useful molecular tools have been developed for its study, there are currently few inducible promoters available for general use. To address this need, we have constructed expression plasmids incorporating upstream elements of the C. neoformans copper transporter gene CTR4, and tested them in C. neoformans serotypes A and D. In response to copper deprivation, these plasmids mediate high-level expression of a reporter protein. This expression can be completely repressed using physiologically low concentrations of copper. Notably, this new family of copper-sensing promoters demonstrates excellent expression in serotype A, contrasting with other available promoters. These plasmids therefore offer efficient and regulated expression for both serotypes A and D, and should be valuable tools for the C. neoformans research community.
新型隐球菌是一种引发严重脑膜炎的机会性真菌病原体。尽管已经开发出许多用于其研究的有用分子工具,但目前可供普遍使用的诱导型启动子却很少。为满足这一需求,我们构建了包含新型隐球菌铜转运蛋白基因CTR4上游元件的表达质粒,并在新型隐球菌A和D血清型中进行了测试。在铜缺乏的情况下,这些质粒介导报告蛋白的高水平表达。使用生理低浓度的铜可以完全抑制这种表达。值得注意的是,这个新的铜感应启动子家族在A血清型中表现出优异的表达,这与其他可用启动子形成对比。因此,这些质粒为A和D血清型提供了高效且受调控的表达,对于新型隐球菌研究群体而言应该是有价值的工具。