从两个季节的“阿尔马格罗”茄子发酵物中分离出的乳酸杆菌的遗传多样性（随机扩增多态性DNA-PCR）

Genetic diversity (RAPD-PCR) of lactobacilli isolated from "Almagro" eggplant fermentations from two seasons.

作者信息

Seseña Susana, Sánchez Isabel, Palop Llanos

机构信息

Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias del Medio Ambiente, Avda. Carlos III s/n, 45071 Toledo, Spain.

出版信息

FEMS Microbiol Lett. 2004 Sep 1;238(1):159-65. doi: 10.1016/j.femsle.2004.07.029.

DOI:10.1016/j.femsle.2004.07.029

PMID:15336417

Abstract

Genetic diversity of 323 strains of lactobacilli isolated from an Almagro eggplant manufacturing plant was analyzed by using random amplified polymorphic DNA (RAPD). Thirty-four distinct RAPD patterns were obtained with 95% of isolates grouped into 18 main clusters. Genetic diversity was higher in brines from season II, Lactobacillus plantarum and Lactobacillus fermentum/cellobiosus being the species with the greatest number of genotypes. A single L. fermentum/cellobiosus genotype comprised isolates from both seasons and could be considered endemic to that factory.

摘要

利用随机扩增多态性DNA（RAPD）分析了从阿尔马格罗茄子制造厂分离出的323株乳酸杆菌的遗传多样性。获得了34种不同的RAPD图谱，95%的分离株被归为18个主要聚类。第二季卤水中的遗传多样性更高，植物乳杆菌和发酵乳杆菌/纤维二糖乳杆菌是基因型数量最多的物种。一个单一的发酵乳杆菌/纤维二糖乳杆菌基因型包含两个季节的分离株，可被认为是该工厂特有的。