Lashchevskiĭ V V, Kovalenko N K
Mikrobiol Z. 2004 Jul-Aug;66(4):3-12.
The numeric analysis of 14 strains of lactobacilli investigated by 57 phenotypical characteristics, has resulted in formation of three clusters: group L. delbrueckii, L. plantarum and the modular group formed by strains of various species. The API-testing, 16S rRNA sequencing and RAPD-typing with primers 5'-AGCAGCGTGG-3' and LP3-ST 5'-TGGTCCGAGC-3' have been carried out for several strains which position in a dendrogram did not correspond to their species diagnosis. The obtained data have allowed the reclassification of the strain L. delbrueckii subsp. bulgaricus B-1923T as L. plantarum B-1923T, L. agilis B-2603 as L. fermentum B-2603, L. amylophilus 30/4/(2) as L. plantarum 30/4/(2). Strain L. confusus 39T, identified by the phenotypical characteristics and data of API-testing as L. fermenturm, was reclassified on the basis of the phylogenetic analysis and RAPD-typing, as L. pentosus 39T.
通过57种表型特征对14株乳酸杆菌进行数值分析，结果形成了三个聚类：德氏乳杆菌组、植物乳杆菌组以及由不同物种菌株组成的模块化组。对一些在聚类图中的位置与其物种诊断不相符的菌株进行了API测试、16S rRNA测序以及使用引物5'-AGCAGCGTGG-3'和LP3-ST 5'-TGGTCCGAGC-3'的RAPD分型。所得数据使得德氏乳杆菌保加利亚亚种B-1923T重新分类为植物乳杆菌B-1923T，敏捷乳杆菌B-2603重新分类为发酵乳杆菌B-2603，嗜淀粉乳杆菌30/4/(2)重新分类为植物乳杆菌30/4/(2)。通过表型特征和API测试数据鉴定为发酵乳杆菌的混淆乳杆菌39T，根据系统发育分析和RAPD分型重新分类为戊糖乳杆菌39T。
