Geraci Fabiana, Pinsino Annalisa, Turturici Guiseppina, Savona Rosalia, Giudice Giovanni, Sconzo Gabriella
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Biochem Biophys Res Commun. 2004 Sep 24;322(3):873-7. doi: 10.1016/j.bbrc.2004.08.005.
Treatment with heavy metals, such as nickel, lead or cadmium, elicits different cellular stress responses according to the metal used and the length of treatment. In Paracentrotus lividus embryos the inducible forms of HSP70 (HSP70/72) are different in molecular mass from the constitutively expressed HSP75, and they can be used as markers of cellular stress. Even a short treatment with each metal induces the synthesis of HSP70/72 which remain stable for at least 20h and differ little in their isoelectric points. Continuous treatment from fertilization with nickel or lead produces late irregular pluteus embryos, with peak HSP70/72 synthesis at blastula followed by the arrest of synthesis by pluteus. On the contrary, the same treatment with cadmium induces continuous HSP70/72 synthesis and produces irregular gastrula embryos which then degenerate. Moreover, a long treatment induces over control embryos a slight increase in the amount of constitutive HSP75 during development while lead treatment depresses constitutive HSP75 at early stages and doubles its quantity at late stages.
用重金属(如镍、铅或镉)进行处理,会根据所使用的金属和处理时间的长短引发不同的细胞应激反应。在紫海胆胚胎中,热休克蛋白70(HSP70/72)的诱导形式在分子量上与组成型表达的热休克蛋白75不同,它们可以用作细胞应激的标志物。即使每种金属进行短时间处理也会诱导HSP70/72的合成,其至少在20小时内保持稳定,并且它们的等电点差异不大。从受精开始用镍或铅进行连续处理会产生晚期不规则的长腕幼虫胚胎,在囊胚期HSP70/72合成达到峰值,随后在长腕幼虫期合成停止。相反,用镉进行相同处理会诱导HSP70/72持续合成,并产生不规则的原肠胚胚胎,然后这些胚胎会退化。此外,长时间处理会导致发育过程中组成型HSP75的量比对照胚胎略有增加,而铅处理在早期会抑制组成型HSP75,在后期使其数量翻倍。