环磷酸腺苷受体蛋白与乳糖操纵子DNA结合时DNA螺旋变化的测量

Measurement of DNA helical change for the binding of cyclic AMP receptor protein to lac DNA.

作者信息

Gang JongBack

机构信息

Department of Chemistry, Kyungwon University, Sungnam, Republic of Korea.

出版信息

Biochem Biophys Res Commun. 2004 Sep 24;322(3):993-7. doi: 10.1016/j.bbrc.2004.08.011.

DOI:10.1016/j.bbrc.2004.08.011

PMID:15336562

Abstract

Cyclic AMP receptor protein (CRP) binds to DNA and induces DNA bending. DNA bending plays an important role in transcription activation of many genes in Escherichia coli. To know DNA helical change by CRP, we used molar cyclization factor (j(M)), which is sensitive to helical twist or torsional alignment. Six consecutive DNA fragments were constructed that contained phasing sequence at left arm and CRP binding site in center. j(M) values measured in this study were approximately 10 times increased by CRP relative to that measured for DNA only. The analysis of set of j(M) values indicated that CRP induced DNA bending without the helical change like helical twisting or torsional alignment change.

摘要

环腺苷酸受体蛋白（CRP）与DNA结合并诱导DNA弯曲。DNA弯曲在大肠杆菌中许多基因的转录激活中起重要作用。为了了解CRP引起的DNA螺旋变化，我们使用了对螺旋扭曲或扭转排列敏感的摩尔环化因子（j(M)）。构建了六个连续的DNA片段，其左臂含有相位序列，中间含有CRP结合位点。本研究中测得的j(M)值相对于仅对DNA测量的值，因CRP而增加了约10倍。对一组j(M)值的分析表明，CRP诱导了DNA弯曲，但没有像螺旋扭曲或扭转排列变化那样的螺旋变化。