缝隙连接蛋白32在慢性肝炎、肝硬化及肝细胞癌中的表达
Expression of gap junction protein connexin32 in chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma.
作者信息
Nakashima Yuichi, Ono Takashi, Yamanoi Akira, El-Assal Osama Nazmy, Kohno Hitoshi, Nagasue Naofumi
机构信息
Second Department of Surgery, Shimane Medical University, Izumo, Japan.
出版信息
J Gastroenterol. 2004 Aug;39(8):763-8. doi: 10.1007/s00535-003-1386-2.
BACKGROUND
The gap junction (GJ) plays important roles in the maintenance of tissue homeostasis, the control of cell growth and differentiation, and the prevention of experimental hepatocarcinogenesis. In this study, we examined the relationship between the expression of the GJ protein connexin (Cx) 32 in 24 human hepatocellular carcinomas (HCCs) and 29 non-carcinomatous liver specimens (NCLs) of 31 patients.
METHODS
An immunohistochemical study of Cx32 was done in 24 HCCs and 29 NCLs from 31 patients who had undergone hepatic resection.
RESULTS
The Cx32 expression decreased gradually as the disease progressed to cirrhosis and HCC. In all Cx32 positive HCCs, the expression was mostly recognized in cytoplasm, not only on the cell membrane. This internalization of Cx32 was also recognized in liver specimens showing hepatitis and cirrhosis, although it was less frequent than in the HCCs.
CONCLUSIONS
These findings suggest the possibility that changes in both the amount and the distribution of Cx32 may be implicated in human hepatocarcinogenesis.
背景
缝隙连接（GJ）在维持组织稳态、控制细胞生长和分化以及预防实验性肝癌发生中发挥重要作用。在本研究中，我们检测了31例患者的24例人类肝细胞癌（HCC）和29例非癌性肝标本（NCL）中GJ蛋白连接蛋白（Cx）32的表达之间的关系。
方法
对31例行肝切除患者的24例HCC和29例NCL进行Cx32的免疫组织化学研究。
结果
随着疾病进展至肝硬化和HCC，Cx32表达逐渐降低。在所有Cx32阳性的HCC中，表达大多见于细胞质，而非仅在细胞膜上。Cx32的这种内化在显示肝炎和肝硬化的肝标本中也可观察到，尽管其频率低于HCC。
结论
这些发现提示Cx32的数量和分布变化可能与人类肝癌发生有关。
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