Ha Eun-Suk, Lee Eun-Ok, Yoon Taek-Joon, Kim Jin-Hyung, Park Jong-Oh, Lim Nak-Cheol, Jung Sung-Ki, Yoon Byung-Soo, Kim Sung-Hoon
Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University, Yongin 449-701, Korea.
Biol Pharm Bull. 2004 Sep;27(9):1348-52. doi: 10.1248/bpb.27.1348.
Spatholobi Caulis has been used in Oriental medicine to treat cancer and blood stasis. In this study, the methylene chloride fraction of Spatholobi Caulis (MCSC) was examined to determine if it possesses anti-cancer activity via its apoptosis-inducing activity. MCSC exhibited a strong cytotoxic effect against human monocyte leukemia U937 cells (IC(50)=15.1 microg/ml). A TUNEL assay showed that the MCSC caused a characteristic ladder pattern of discontinuous DNA fragments and apoptotic bodies. Flow cytometric analysis confirmed that MCSC significantly increases the number of apoptotic cells stained by annexin V(+)/PI(-) cells. Western blotting revealed that MCSC activated caspase-3 expression and cleaved poly (ADP-ribose) polymerase (PARP) in a concentration-dependent manner. An enzyme-linked immunosorbent assay (ELISA) demonstrated that MCSC significantly activated the caspase-3 activity compared with the untreated control by. Taken together, these results suggest that MCSC can induce apoptosis in U937cells via the caspase dependent pathway.
鸡血藤在东方医学中被用于治疗癌症和血瘀。在本研究中，对鸡血藤的二氯甲烷提取物（MCSC）进行了检测，以确定其是否通过诱导凋亡活性而具有抗癌活性。MCSC对人单核细胞白血病U937细胞表现出强烈的细胞毒性作用（IC(50)=15.1微克/毫升）。TUNEL检测显示，MCSC导致了不连续DNA片段和凋亡小体的特征性梯状模式。流式细胞术分析证实，MCSC显著增加了膜联蛋白V(+)/PI(-)细胞染色的凋亡细胞数量。蛋白质免疫印迹法显示，MCSC以浓度依赖的方式激活了caspase-3的表达并切割了聚（ADP-核糖）聚合酶（PARP）。酶联免疫吸附测定（ELISA）表明，与未处理的对照相比，MCSC显著激活了caspase-3的活性。综上所述，这些结果表明，MCSC可通过caspase依赖途径诱导U937细胞凋亡。
