Guo Yuan-ji, Wen Le-ying, Wang Min, Guo Jun-feng, Zhang Ye, Li Zi
Department of Influenza, National Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Sep;17(3):225-8.
To understand the characteristics of internal genes of two strains of influenza A(H9N2) virus isolated from men and on the basis of these to reveal the origin of these two strains of influenza A(H9N2)virus.
The target gene was amplified by RT-PCR,the PCR product was ligated with P GEM-T Vector (Promega Company, USA) at 4 degrees, the recombined plasmid was transferred into dH5a bacteria, and the positive colonies were selected and identified with restriction enzyme. Afterwards, they were sent to Liu He Tong Company in Beijing for nucleotide sequencing. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (version 1.03)and Editseq (Version 3.69) softwares.
Internal genes of the two strains of H9N2 virus were G9 lineage. There was a slight difference in nucleotide sequence in PA gene between the two strains, whereas another five gene segments were identical to each other.
The genomes of the two strains of influenza A(H9N2)virus were G9 lineage.They were transmitted to men separately from different avian sources with different characteristics of gene of influenza A(H9N2) virus, respectively.
了解从人分离出的两株甲型H9N2流感病毒内部基因特征，并据此揭示这两株甲型H9N2流感病毒的来源。
采用RT-PCR扩增目的基因，PCR产物于4℃与PGEM-T载体（美国Promega公司）连接，重组质粒转入DH5α菌，筛选阳性菌落并用限制性内切酶进行鉴定。之后送北京六合通公司进行核苷酸测序。最后用MegAlign（版本1.03）和Editseq（版本3.69）软件对测序数据进行系统发育分析。
两株H9N2病毒的内部基因为G9谱系。两株病毒PA基因的核苷酸序列存在细微差异，而其他五个基因片段彼此相同。
两株甲型H9N2流感病毒基因组均为G9谱系。它们分别从不同禽类来源以甲型H9N2流感病毒不同的基因特征分别传播给人。
