[通过噬菌体展示文库产生的人抗人巨细胞病毒中和Fab抗体]
[Human anti-HCMV neutralizing Fab antibody generated by phage display library].
作者信息
Duan Tao, Liang Mi-fang, Gu Shu-yan
机构信息
Virus Emergency Biotech Center, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Sep;17(3):247-50.
BACKGROUND
Cloning recombinant human Fab fragment against HCMV for the purpose of prophylaxis and control of HCMV infection.
METHODS
The authors constructed a HCMV phage display library with 2 x 10(6) clones, then used purified HCMV viral lysates to pan the library, then screened by ELISA.
RESULTS
Three clones showed positive responses in ELISA, they also showed high specificity in IFA, two of them could neutralize HCMV in neutralizing assays.
CONCLUSION
The specific binding of Fab antibodies to HCMV was demonstrated by ELISA, IFA and neutralizing activities. These results provide us the basis for further research of neutralizing recombinant human whole IgG molecule.
背景
克隆针对人巨细胞病毒（HCMV）的重组人Fab片段，用于预防和控制HCMV感染。
方法
作者构建了一个具有2×10⁶个克隆的HCMV噬菌体展示文库，然后用纯化的HCMV病毒裂解物对文库进行淘选，接着通过酶联免疫吸附测定（ELISA）进行筛选。
结果
三个克隆在ELISA中显示出阳性反应，它们在间接免疫荧光法（IFA）中也显示出高特异性，其中两个在中和试验中能够中和HCMV。
结论
通过ELISA、IFA和中和活性证明了Fab抗体与HCMV的特异性结合。这些结果为进一步研究中和重组人全IgG分子提供了基础。
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