Sun Xiao Dong, Yu Zuo Ren, Ma Jing, Ge Ye Hua, Li Sai, Xue She Pu, Han Dai Shu
Department of Cell Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005.
Shi Yan Sheng Wu Xue Bao. 2002 Mar;35(1):21-5.
The centrosomal protein, Cenexin, is a molecular marker of mature centriole. To elucidate the variability and function of mature centriole in spermatogenesis, the high titer polyclonal antibody against rat cenexin was obtained by immunizing mice with recombinant cenexin which was made up in this study. The expression of cenexin in rat spermatogenesis was carried out by semi-quantitative RT-PCR, immunofluorescence and Western Blot. The results demonstrated that the level of Cenexin mRNA was higher in spermatogonia and spermatocytes, then decreased in following stages, while Cenexin protein was located on one centriole from spermatogonia to spermatids, showing mature centriole existed in these stages. Cenexin protein was localized to the basal body of the flagellum in elongated spermatids and the stained signal disappeared in the most of epidydimal sperms. These results suggested that the expression pattern of cenexin in rat spermatogenesis might be related to the initiation of the flagella formation.
中心体蛋白Cenexin是成熟中心粒的分子标记。为了阐明成熟中心粒在精子发生过程中的变异性和功能，本研究通过用重组Cenexin免疫小鼠，获得了效价高的抗大鼠Cenexin多克隆抗体。采用半定量RT-PCR、免疫荧光和蛋白质免疫印迹法检测大鼠精子发生过程中Cenexin的表达。结果表明，Cenexin mRNA水平在精原细胞和精母细胞中较高，在随后的阶段降低，而Cenexin蛋白从精原细胞到精子细胞均定位于一个中心粒上，表明这些阶段存在成熟中心粒。在长形精子细胞中，Cenexin蛋白定位于鞭毛的基体，在大多数附睾精子中染色信号消失。这些结果提示，Cenexin在大鼠精子发生中的表达模式可能与鞭毛形成的起始有关。
