Liu JingMei, Chen DaMing, Xu Yong, Chen Hang
Vegetable Research Center of Beijing Acadamy of Agriculture and Forest Science, Beijing 100089.
Shi Yan Sheng Wu Xue Bao. 2002 Mar;35(1):31-5.
The 5' flanking region of ADP-Glucose Pyrophosphorylase large subunit gene wml1, which covers 1864 bp, was isolated from watermelon genomic DNA by Uneven PCR. Sequence analysis indicated that there were putative TATA box and CAAT box at position 1237bp and 1284bp of the cloned fragment, respectively. Two transcriptional fusions between 5' regulational sequence of wml1 and beta-glucuronidase gene were constructed. Constructs containing 180bp-1752bp fragment could drive GUS gene transient express specifically in watermelon fruit, while 958bp-1752bp had no promoter activity. Putative fruit-specific cis-acting elements may locate between 180bp and 958bp.
通过不对称PCR从西瓜基因组DNA中分离出ADP-葡萄糖焦磷酸化酶大亚基基因wml1的5'侧翼区域，该区域长度为1864 bp。序列分析表明，在克隆片段的1237 bp和1284 bp位置分别存在推定的TATA盒和CAAT盒。构建了wml1的5'调控序列与β-葡萄糖醛酸酶基因之间的两种转录融合体。含有180 bp - 1752 bp片段的构建体可驱动GUS基因在西瓜果实中特异性瞬时表达，而958 bp - 1752 bp则没有启动子活性。推定的果实特异性顺式作用元件可能位于180 bp和958 bp之间。
