Nikitina T V, Nazarova N Y, Aksenov N D, Tishchenko L I, Tuohimaa P, Sedova V M
Department of Biochemistry, St. Petersburg State University.
Tsitologiia. 2004;46(5):437-41.
The level of 5S rRNA and tRNAi(Met)1 synthesized by RNA polymerase III was investigated in human epidermoid carcinoma cells A431 at different physiological states: low and high proliferation and apoptosis. The real-time RT-PCR method using SYBR Green I was applied to measure certain RNA species in total cellular RNA. The share of 5S rRNA was practically the same in slowly and actively proliferating A431 cells, but increased about 2.5-fold in apoptotic cells. The share of initiator tRNAi(Met)1 in actively proliferating and apoptotic cells was 1.5-2.0 times higher than in slowly proliferating cells. Our results suggest a possible existence of special mechanisms regulating RNA polymerase III-directed transcription from different type promoters in accordance with the physiological state of the cell.
在处于不同生理状态（低增殖和高增殖以及凋亡）的人表皮样癌细胞A431中，研究了由RNA聚合酶III合成的5S rRNA和起始tRNAi(Met)1的水平。采用SYBR Green I的实时RT-PCR方法用于测量总细胞RNA中的某些RNA种类。在缓慢增殖和活跃增殖的A431细胞中，5S rRNA的占比实际上相同，但在凋亡细胞中增加了约2.5倍。起始tRNAi(Met)1在活跃增殖细胞和凋亡细胞中的占比比缓慢增殖细胞高1.5 - 2.0倍。我们的结果表明，可能存在特殊机制，可根据细胞的生理状态调节RNA聚合酶III从不同类型启动子进行的转录。
