Martynova M G, Krylova M I, Bystrova O A
Tsitologiia. 2004;46(5):448-55.
In parallel with contraction, vertebrate cardiomyocytes perform endocrine function and produce natriuretic peptides (NP)--ANP and BNP--involved in cardiovascular homeostasis maintenance. ANP-like peptides have been reported also in hearts of some invertebrates, however, their cellular localization was not determined. By electron microscopical immunocytochemistry with polyclonal monospecific antibodies raised against ANP and protein A-gold technique, we have localized ANP-like immunoreactivity in granules within endothelial cells in the heart of the brachiopod Rhynchonella psittacea, the polychaete Arenicola marina, and the gastropod mollusc Achatina fulica--all being representatives of the major phylogenetic group Lophotrochozoa. ANP-like immunoreactivity was also revealed in one of 3 morphologically distinguishable types of granules in the snail heart granular cells. By electron microscopical autoradiography with the use of [3H]-thymidine, the ability for DNA synthesis was demonstrated in heart endothelial cells of the investigated animals. Forms of NP-system organization in hearts of Lophotrochozoa and Vertebrates, and close histogenetic relationships of endothelial and granular cells in the snail heart are discussed.
与收缩同时，脊椎动物心肌细胞执行内分泌功能并产生参与维持心血管稳态的利钠肽（NP）——心房钠尿肽（ANP）和脑钠肽（BNP）。在一些无脊椎动物的心脏中也报道了类似ANP的肽，然而，它们的细胞定位尚未确定。通过使用针对ANP产生的多克隆单特异性抗体和蛋白A-金技术进行电子显微镜免疫细胞化学，我们在腕足动物鹦鹉贝（Rhynchonella psittacea）、多毛纲动物沙蠋（Arenicola marina）和腹足纲软体动物非洲大蜗牛（Achatina fulica）（所有这些都是主要系统发育类群冠轮动物的代表）的心脏内皮细胞内的颗粒中定位了类似ANP的免疫反应性。在蜗牛心脏颗粒细胞中3种形态上可区分的颗粒类型之一中也发现了类似ANP的免疫反应性。通过使用[3H] - 胸腺嘧啶进行电子显微镜放射自显影，证明了所研究动物的心脏内皮细胞具有DNA合成能力。本文讨论了冠轮动物和脊椎动物心脏中NP系统的组织形式，以及蜗牛心脏中内皮细胞和颗粒细胞之间密切的组织发生关系。
