Asano Haruhiko, Murate Takashi, Naoe Tomoki, Saito Hidehiko, Stamatoyannopoulos George
Department of Hematology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya, Japan 466-8550.
Biochem J. 2004 Dec 15;384(Pt 3):647-53. doi: 10.1042/BJ20040394.
We have cloned a gene, ZFF29 (zinc-finger protein of human fetal liver erythroid cells 29), from human fetal liver erythroid cells. Two types of mature mRNA were identified and designated ZFF29a and ZFF29b. In human genome the ZFF29 gene is on chromosome 9q, and the two forms are splice variants. There is a unique transcription start site, which predicts major mRNAs composed of 2485 bases for ZFF29a and 1801 bases for ZFF29b. The anticipated mRNAs were demonstrated in K562 cells, but not in any adult human tissues examined by Northern blotting. In the mouse, reverse transcription-PCR revealed that the ZFF29 mRNA is present in adult bone marrow and ovary at a higher level than in any other tissues examined. These findings suggest that ZFF29 proteins are expressed in embryonic/fetal erythroid tissues. The deduced polypeptide chains of ZFF29a and ZFF29b are composed of 306 and 350 amino acids respectively. A unique zinc-finger motif composed of two contiguous Cys(2)His(2)-type fingers is common to both forms of ZFF29. They are nuclear proteins and ZFF29b, but not ZFF29a, is an activator of erythroid gene promoters.
我们从人胎儿肝脏红系细胞中克隆了一个基因ZFF29（人胎儿肝脏红系细胞锌指蛋白29）。鉴定出两种成熟的mRNA，分别命名为ZFF29a和ZFF29b。在人类基因组中，ZFF29基因位于9号染色体q区，这两种形式是剪接变体。有一个独特的转录起始位点，预测ZFF29a的主要mRNA由2485个碱基组成，ZFF29b的主要mRNA由1801个碱基组成。预期的mRNA在K562细胞中得到证实，但在通过Northern印迹检测的任何成人组织中均未检测到。在小鼠中，逆转录PCR显示ZFF29 mRNA在成年骨髓和卵巢中的表达水平高于所检测的任何其他组织。这些发现表明ZFF29蛋白在胚胎/胎儿红系组织中表达。ZFF29a和ZFF29b推导的多肽链分别由306和350个氨基酸组成。由两个相邻的Cys(2)His(2)型锌指组成的独特锌指基序在ZFF29的两种形式中都很常见。它们是核蛋白，并且ZFF29b而非ZFF29a是红系基因启动子的激活剂。
