Liu Daorong, Tammik Charlotte, Zou Jie-Zhi, Ernberg Ingemar, Masucci Maria G, Ringden Olle, Levitsky Victor
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
Clin Transplant. 2004 Oct;18(5):518-24. doi: 10.1111/j.1399-0012.2004.00198.x.
Recipients of T-cell-depleted bone marrow (BM) transplants (BMT) frequently develop Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) preceded by a rapid and prominent increase of EBV load in the peripheral blood. The level of this increase positively correlates with the incidence of PTLD. Using a semiquantitative PCR assay we compared the blood levels of EBV-DNA in patients transplanted with either T-cell or T- and B-cell-depleted human leukocyte antigen (HLA)-mismatched BM grafts. Combined T- and B-cell depletion correlated with significantly lower maximal levels of EBV load, which were reached with slower kinetics. These data indicate that B-cell depletion of BM can be used for prophylaxis of PTLD in BM transplant recipients and can affect the long-term balance between EBV and its host.
接受去除T细胞的骨髓（BM）移植（BMT）的患者经常会发生与爱泼斯坦-巴尔病毒（EBV）相关的移植后淋巴细胞增生性疾病（PTLD），在此之前外周血中EBV载量会迅速且显著增加。这种增加的水平与PTLD的发病率呈正相关。我们使用半定量PCR检测法比较了接受T细胞或T细胞和B细胞均去除的人类白细胞抗原（HLA）不匹配BM移植物的患者血液中EBV-DNA的水平。T细胞和B细胞联合去除与显著更低的EBV载量最大水平相关，且达到这一水平的动力学较慢。这些数据表明，BM的B细胞去除可用于预防BM移植受者的PTLD，并可影响EBV与其宿主之间的长期平衡。
