Vijayvergiya Viksita, Wilson Ryan, Chorak Adam, Gao Philip Fei, Cross Timothy A, Busath David D
Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah 84602, USA.
Biophys J. 2004 Sep;87(3):1697-704. doi: 10.1529/biophysj.104.043018.
Purified M2 protein from the Udorn strain of influenza virus was reconstituted into planar lipid bilayers from liposomes. In 1 mM HCl, the single-channel conductance was measured as 6 pS with open probability of < or =0.03. The current voltage curve is linear over the achievable voltage range. The current amplitude is amantadine sensitive. In HCl solutions, the single-channel current was essentially invariant with changes in [Cl(-)], [Na(+)], and [tetraethylammonium] ([TEA(+)]), but dependent on [H(+)]. The reversal potential, determined with asymmetrical hydrogen chloride solution, is very close to the equilibrium potential of hydrogen. This appears to be the first report of single-channel proton currents with the full-length M2 protein.
从流感病毒乌多恩株中纯化的M2蛋白被重新整合到脂质体形成的平面脂质双分子层中。在1 mM盐酸中，单通道电导测量为6 pS，开放概率≤0.03。电流电压曲线在可达到的电压范围内呈线性。电流幅度对金刚烷胺敏感。在盐酸溶液中，单通道电流基本上不随[Cl⁻]、[Na⁺]和四乙铵的变化而变化，但依赖于[H⁺]。用不对称氯化氢溶液测定的反转电位非常接近氢的平衡电位。这似乎是关于全长M2蛋白单通道质子电流的首次报道。
