Yang Peixin, Wang Jinrong, Shen Yulei, Roy Shyamal K
Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, Nebraska 68198-4515, USA.
Endocrinology. 2004 Dec;145(12):5757-66. doi: 10.1210/en.2004-0779. Epub 2004 Sep 2.
Perinatal expression of estrogen receptor (ER) protein and mRNA and the influence of FSH on this process were examined by immunofluorescence and RT-PCR using ovaries from fetal (d 13-15 of gestation) and postnatal [postnatal d 1-15 (P1-P15)] hamsters and from 8-d-old hamsters exposed in utero to an anti-FSH serum on d 12 of gestation and saline or equine chorionic gonadotropin (eCG) on P1. A few somatic cells expressing ERalpha immunoreactivity appeared first on d 14 of gestation and increased markedly by P8-P15 in the interstitial cells and granulosa cells of primordial follicles. In contrast, appreciable ERbeta immunoreactivity was localized on d 13 of gestation, and more cells expressed ERbeta immunoreactivity by P1-P8. By P7, ERbeta immunoreactivity was present in cells adjacent to the oocytes, and by P8, ERbeta was preferentially localized in the granulosa cells. Receptor immunoreactivities decreased markedly in P8 ovaries exposed in utero to the FSH antiserum but were reversed with postnatal eCG replacement. Oocytes and somatic cells expressed ERalpha and ERbeta mRNA, and levels of ER mRNA in the ovary increased by P7-P8, corresponding to the appearance of primordial follicles. Thereafter, only ERbeta mRNA levels increased progressively with postnatal ovary development. Similar to ER protein, mRNA levels decreased significantly in FSH antiserum-treated ovaries but were restored by eCG. These results indicate that both ER subtypes are expressed in undifferentiated somatic cells and the oocytes during perinatal ovary development in the hamster; however, ERbeta expression segregates with the differentiation of granulosa cells. Furthermore, ER expression and differentiation of somatic cells to granulosa cells depend on perinatal FSH action.
通过免疫荧光和逆转录聚合酶链反应（RT-PCR），利用来自胎儿（妊娠第13 - 15天）和出生后[出生后第1 - 15天（P1 - P15）]仓鼠的卵巢，以及来自在妊娠第12天子宫内暴露于抗促卵泡激素（FSH）血清且在出生后第1天接受生理盐水或马绒毛膜促性腺激素（eCG）处理的8日龄仓鼠的卵巢，检测雌激素受体（ER）蛋白和mRNA的围产期表达以及FSH对这一过程的影响。一些表达ERα免疫反应性的体细胞最早出现在妊娠第14天，并在出生后第8 - 15天在原始卵泡的间质细胞和颗粒细胞中显著增加。相比之下，可观的ERβ免疫反应性在妊娠第13天定位，并且到出生后第1 - 8天有更多细胞表达ERβ免疫反应性。到出生后第7天，ERβ免疫反应性存在于与卵母细胞相邻的细胞中，到出生后第8天，ERβ优先定位于颗粒细胞中。在子宫内暴露于FSH抗血清的出生后第8天卵巢中，受体免疫反应性显著降低，但出生后用eCG替代可使其逆转。卵母细胞和体细胞表达ERα和ERβ mRNA，卵巢中ER mRNA水平在出生后第7 - 8天增加，与原始卵泡的出现相对应。此后，只有ERβ mRNA水平随着出生后卵巢发育而逐渐增加。与ER蛋白相似，在FSH抗血清处理的卵巢中mRNA水平显著降低，但通过eCG可恢复。这些结果表明，在仓鼠围产期卵巢发育过程中，两种ER亚型均在未分化的体细胞和卵母细胞中表达；然而，ERβ表达与颗粒细胞的分化相关。此外，ER表达以及体细胞向颗粒细胞的分化依赖于围产期FSH的作用。
