链脲佐菌素处理大鼠肝脏和肝细胞膜中抑制性鸟嘌呤核苷酸调节蛋白（Gi）功能的证明。
Demonstration of inhibitory guanine nucleotide regulatory protein (Gi) function in liver and hepatocyte membranes from streptozotocin-treated rats.
作者信息
Kirkham D M, Murphy G J, Young P
机构信息
SmithKline Beecham Pharmaceuticals, Research Division, Epsom, Surrey, U.K.
出版信息
Biochem J. 1992 Jun 1;284 ( Pt 2)(Pt 2):301-4. doi: 10.1042/bj2840301.
Abstract
By using a defined plasma-membrane preparation, functional inhibition of adenylate cyclase activity by the inhibitory G-protein (Gi) was observed in liver and hepatocyte membranes from rats made diabetic by streptozotocin. These observations contrast with previous reports which have shown a defect in Gi in this diabetic animal model. These results suggest that Gi function is not impaired in the livers of streptozotocin-treated rats and that plasma-membrane preparation procedures should be clearly defined before ascribing Gi defects to a pathological state such as diabetes.
摘要
通过使用特定的质膜制剂，在链脲佐菌素诱导的糖尿病大鼠的肝脏和肝细胞膜中观察到抑制性G蛋白（Gi）对腺苷酸环化酶活性的功能性抑制。这些观察结果与之前报道的在该糖尿病动物模型中Gi存在缺陷形成对比。这些结果表明，在链脲佐菌素处理的大鼠肝脏中，Gi功能并未受损，并且在将Gi缺陷归因于糖尿病等病理状态之前，应明确质膜制备程序。
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