Cha Jae-Young, Park Jin-Chul, Jeon Beong-Sam, Lee Young-Choon, Cho Young-Su
Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
J Microbiol. 2004 Mar;42(1):51-5.
The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.
研究了原料氨基酸、盐、碳源和氮源对酿酒酵母FF-8生产谷胱甘肽的影响。发现葡萄糖、酵母提取物、KH2PO4和L-半胱氨酸是合适的原料。在含有3.0%（w/v）葡萄糖、3.0%酵母提取物、0.06% KH2PO4和0.06% L-半胱氨酸的培养基中振荡培养72小时后，谷胱甘肽产量最高。与YM基础培养基相比，使用该培养基获得的谷胱甘肽浓度增加了2.27倍，达到204 mg/l。
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