Schmacht Maximilian, Lorenz Eric, Stahl Ulf, Senz Martin
Research and Teaching Institute for Brewing in Berlin, Department Bioprocess Engineering and Applied Microbiology, Seestrasse 13, 13353 Berlin, Germany.
Berlin University of Technology, Institute of Biotechnology, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
J Biosci Bioeng. 2017 May;123(5):555-561. doi: 10.1016/j.jbiosc.2016.12.011. Epub 2017 Jan 12.
The production of glutathione (GSH) or GSH enriched yeast is still in the focus of research driven by a high industrial interest. In this study, an optimal growth rate for GSH production via Saccharomyces cerevisiae Sa-07346 was investigated. To further improve the fermentation process in a way that it is independent of lots, the influence of different WMIX medium compositions on biomass and GSH production was studied. Thereby, the fermentation medium was adjusted based on yeast's elemental composition. The resulting chemically defined fermentation medium led to high cell densities in fed-batches. Therefore, it has the potential to be applied successfully for other high cell density yeast fermentation processes. As cysteine is the key component for GSH production, different cysteine addition strategies were studied and finally, a continuous cysteine feeding was applied in the late stage of fermentation. Thereby, a GSH concentration of 1459 ± 57 mg/l was reached by continuously feeding cysteine, which meant an increase to 253% compared to the control without cysteine addition (577 mg/l GSH).
由于具有很高的工业价值，谷胱甘肽（GSH）或富含GSH的酵母的生产仍然是研究的重点。在本研究中，研究了酿酒酵母Sa - 07346生产GSH的最佳生长速率。为了以一种与批次无关的方式进一步改进发酵过程，研究了不同WMIX培养基组成对生物量和GSH生产的影响。由此，根据酵母的元素组成调整了发酵培养基。所得的化学限定发酵培养基在补料分批培养中导致了高细胞密度。因此，它有潜力成功应用于其他高细胞密度酵母发酵过程。由于半胱氨酸是GSH生产的关键成分，研究了不同的半胱氨酸添加策略，最终在发酵后期采用了连续半胱氨酸补料。通过连续补料半胱氨酸，GSH浓度达到了1459±57mg/l，与不添加半胱氨酸的对照（577mg/l GSH）相比增加到了253%。
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