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甜菜中的超氧化物歧化酶转基因赋予对氧化试剂和甜菜生尾孢菌的抗性。

Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C. beticola.

作者信息

Tertivanidis Konstantinos, Goudoula Catherine, Vasilikiotis Christos, Hassiotou Efthymia, Perl-Treves Rafael, Tsaftaris Athanasios

机构信息

Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 261, Thessaloniki 540 06, Greece.

出版信息

Transgenic Res. 2004 Jun;13(3):225-33. doi: 10.1023/b:trag.0000034610.35724.04.

Abstract

Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of sugarbeet petioles. The transgenic plants were subjected to treatments known to cause oxidative stress, such as the herbicide methyl viologen and a natural photosensitizer toxin produced by the fungus Cercospora beticola, namely cercosporin. The transgenic plants exhibited increased tolerance to methyl viologen, to pure cercosporin, as well as to leaf infection with the fungus C. beticola.

摘要

为了研究增强甜菜对氧化应激抗性的可能性,培育了携带超氧化物歧化酶转基因的甜菜。携带来自番茄的叶绿体和胞质超氧化物歧化酶基因的双元T-DNA载体,用于农杆菌介导的甜菜叶柄转化。对转基因植物进行已知会引起氧化应激的处理,如除草剂甲基紫精和由甜菜尾孢菌产生的天然光敏毒素即尾孢菌素。转基因植物对甲基紫精、纯尾孢菌素以及甜菜尾孢菌的叶片感染表现出更高的耐受性。

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