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超氧化物歧化酶的全基因组鉴定及其在果实发育和生物胁迫响应中的表达:一项生物信息学研究

Genome-Wide Identification of Superoxide Dismutase and Expression in Response to Fruit Development and Biological Stress in : A Bioinformatics Study.

作者信息

Yang Huai, Zhang Qiuyi, Zhong Shengfu, Yang Hao, Ren Tianheng, Chen Chen, Tan Feiquan, Cao Guoxing, Liu Jun, Luo Peigao

机构信息

Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University of Sichuan Province, Chengdu 611130, China.

College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Antioxidants (Basel). 2023 Mar 15;12(3):726. doi: 10.3390/antiox12030726.

Abstract

is a newly domesticated perennial fruit tree, and the lack of molecular research on stress resistance seriously affects its genetic improvement and commercial value development. Superoxide dismutase () can effectively eliminate the accumulation of reactive oxygen species (ROS) during the rapid growth of plant organs under biotic and abiotic stresses, maintaining a steady state of physiological metabolism. In this study, 13 consisting of two (), four () and seven () were identified in the genome. Structurally, the phylogeny, intron-exon pattern and motif sequences within these three subfamilies show high conservation. Evolutionarily, segmental/wide genome duplication (WGD) and dispersed duplication form the current profile of . Weighted gene coexpression network analysis (WGCNA) revealed the metabolic pathways of nine (69.2%) involved in fruit development, among which regulates fruit development and participates in the stress response. In addition, under the stress of multiple pathogens, six (46.6%) were continuously upregulated in the rinds of resistant lines; of these, three (, and ) were weakly or not expressed in susceptible lines. The results pave the way for theoretical research on and afford the opportunity for genetic improvement of .

摘要

是一种新驯化的多年生果树,而缺乏对抗逆性的分子研究严重影响其遗传改良和商业价值开发。超氧化物歧化酶()能够在生物和非生物胁迫下植物器官快速生长期间有效消除活性氧(ROS)的积累,维持生理代谢的稳定状态。在本研究中,在基因组中鉴定出13个,由两个()、四个()和七个()组成。在结构上,这三个亚家族内的系统发育、内含子-外显子模式和基序序列显示出高度保守性。在进化上,片段/全基因组复制(WGD)和分散复制形成了当前的概况。加权基因共表达网络分析(WGCNA)揭示了九个(69.2%)参与果实发育的代谢途径,其中调节果实发育,参与胁迫反应。此外,在多种病原体胁迫下,抗性品系果皮中有六个(46.6%)持续上调;其中,三个(、和)在感病品系中弱表达或不表达。这些结果为的理论研究铺平了道路,并为的遗传改良提供了机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d5/10045841/2cc346cfe500/antioxidants-12-00726-g001.jpg

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