Velickovic Z M, Dodd R, Velickovic M, Hersee J, Le T, Taverniti A, Wallace R, Dunckley H
Molecular Genetics, Tissue Typing, Australian Red Cross Blood Service, Sydney, Australia.
Tissue Antigens. 2004 Oct;64(4):509-11. doi: 10.1111/j.1399-0039.2004.00288.x.
Three new human leukocyte antigen (HLA) class I alleles have been identified in the Tissue Typing Laboratory in Sydney, Australia. Sequence analysis of exon 2 and exon 3 of the HLA-B gene revealed the novel polymorphism. A silent substitution of C to T at nucleotide position 369 has been identified for the HLA-B400104 allele when compared to the closest matched allele, HLA-B400101. The HLA-B3928 allele was identified with a nucleotide substitution of G to C at position 362 when compared to the closest matched allele, HLA-B390101, resulting in an amino acid substitution of Arginine to Threonine. A nucleotide substitution of C to G at position 572 resulting in the amino acid change Serine to Tryptophan was identified in the new allele HLA-B4437, when compared to the closest matched allele HLA-B440301. Both amino acid substitutions implicate a different specificity and affinity of antigen binding for the alleles HLA-B3928 and HLA-B4437.
在澳大利亚悉尼的组织分型实验室中，已鉴定出三种新的人类白细胞抗原（HLA）I类等位基因。对HLA - B基因的外显子2和外显子3进行序列分析，揭示了这种新的多态性。与最匹配的等位基因HLA - B400101相比，HLA - B400104等位基因在核苷酸位置369处存在C到T的沉默替换。与最匹配的等位基因HLA - B390101相比，HLA - B3928等位基因在位置362处存在G到C的核苷酸替换，导致精氨酸到苏氨酸的氨基酸替换。与最匹配的等位基因HLA - B440301相比，在新等位基因HLA - B4437中鉴定出在位置572处C到G的核苷酸替换，导致丝氨酸到色氨酸的氨基酸变化。这两种氨基酸替换都表明HLA - B3928和HLA - B4437等位基因的抗原结合具有不同的特异性和亲和力。
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