[一种新型HLA等位基因HLA-B*9526的鉴定]

[Identification of a novel HLA allele HLA-B*9526].

作者信息

Chen Yang, Liu Xianzhi, Li Xiaofeng, Zhang Xu, Zhang Kunlian, Li Jianping

机构信息

Liaoning Blood Center, Shenyang, Liaoning, 110044 PR China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Dec;26(6):705-8. doi: 10.3760/cma.j.issn.1003-9406.2009.06.021.

DOI:10.3760/cma.j.issn.1003-9406.2009.06.021

PMID:19953499

Abstract

OBJECTIVE

A novel human leukocyte antigen-B (HLA-B) allele, B*9526, was identified and analyzed by sequencing-based method in a Chinese donor.

METHODS

HLA typing was performed by PCR-sequence-specific oligonucleotide (SSO). Molecular cloning and DNA sequencing were used to identify the sequence of the potential novel allele and the difference between this new allele and other known alleles were analyzed.

RESULTS

HLA genotyping of one sample gave different results. The sequencing result showed HLA-B alleles of the proband as B5403 and a novel allele. The nucleotide sequence of the novel allele was different from all known B alleles and harbored one nucleotide change from the closest matching allele B1507 at nucleotide 425 (A to G) in exon 3, resulting in an amino acid change from Y (TAC) to C (TGC) at codon 142.

CONCLUSION

A novel HLA allele was identified and officially designated as HLA-B*9526 by WHO Nomenclature Committee for Factors of the HLA System.

摘要

目的

通过基于测序的方法在中国一名供者中鉴定并分析了一种新的人类白细胞抗原B（HLA - B）等位基因，即B*9526。

方法

采用聚合酶链反应 - 序列特异性寡核苷酸（PCR - SSO）进行HLA分型。运用分子克隆和DNA测序来鉴定潜在新等位基因的序列，并分析该新等位基因与其他已知等位基因之间的差异。

结果

一个样本的HLA基因分型得出了不同结果。测序结果显示先证者的HLA - B等位基因为B5403和一个新等位基因。该新等位基因的核苷酸序列与所有已知的B等位基因均不同，在第3外显子的425位核苷酸处与最匹配的等位基因B1507有一个核苷酸变化（A变为G），导致第142密码子处的氨基酸从Y（TAC）变为C（TGC）。