Nell Marja J, Tjabringa G Sandra, Vonk Marcel J, Hiemstra Pieter S, Grote Jan J
Department of Otolaryngology, Leiden University Medical Center, Albinusdreef 2, Room J2-77, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
FEMS Immunol Med Microbiol. 2004 Oct 1;42(2):225-31. doi: 10.1016/j.femsim.2004.05.013.
The respiratory epithelium plays a major role in the primary defense of the airways against infection. It has been demonstrated that bacterial products are involved in the induction of inflammatory reactions of the upper airways. Little is known about the effects of bacterial products on expression of the antimicrobial peptide hCAP-18/LL-37, the only human cathelicidin identified so far. The aim of this study was to investigate the effects of bacterial products from both gram-positive and gram-negative bacteria on the expression of hCAP-18/LL-37 by sinus epithelial cells using an air-exposed tissue culture model. Lipopolysaccharide and lipoteichoic acid both increased hCAP-18/LL-37 expression in cultured sinus epithelium as assessed by immunohistochemistry, where maximal stimulation occurred at 100 ng ml(-1) lipopolysaccharide or 10 microg ml(-1) lipoteichoic acid. The stimulatory effect of lipopolysaccharide and lipoteichoic acid was not restricted to expression of hCAP-18/LL-37, since also mucin expression and IL-8 release from cultured sinus epithelium cells were increased by lipopolysaccharide and lipoteichoic acid. This suggests that bacterial products may stimulate innate immunity in the upper airways.
呼吸道上皮在气道抵御感染的初级防御中起主要作用。已证实细菌产物参与上呼吸道炎症反应的诱导。关于细菌产物对抗菌肽hCAP-18/LL-37（迄今唯一鉴定出的人源cathelicidin）表达的影响知之甚少。本研究的目的是使用空气暴露组织培养模型，研究革兰氏阳性菌和革兰氏阴性菌的细菌产物对鼻窦上皮细胞hCAP-18/LL-37表达的影响。通过免疫组织化学评估，脂多糖和脂磷壁酸均增加了培养的鼻窦上皮中hCAP-18/LL-37的表达，其中在100 ng/ml脂多糖或10 μg/ml脂磷壁酸时出现最大刺激。脂多糖和脂磷壁酸的刺激作用不仅限于hCAP-18/LL-37的表达，因为脂多糖和脂磷壁酸也增加了培养的鼻窦上皮细胞中粘蛋白的表达和IL-8的释放。这表明细菌产物可能刺激上呼吸道的固有免疫。
