Ni Hong, Jiang Yu-Wu, Bo Tao, Wang Jing-Min, Pan Hong, Wu Xi-Ru
Department of Pediatrics, Peking University First Hospital, No. 8 Xishiku Street, 100034 Beijing, PR China.
Neurosci Lett. 2004 Sep 30;368(3):254-7. doi: 10.1016/j.neulet.2004.05.008.
To evaluate the pathophysiological mechanism of subsequent reduced seizure threshold following neonatal seizures, single or recurrent prolonged seizures were induced to neonatal rats by the inhalant flurothyl. The expression of N-methyl-d-aspartate receptor 1 (NR1) and gamma-aminobutyric acid receptor A-alpha 1 (GABA-A-alpha 1) immunoreactivity in hippocampus were examined by Western blotting analysis at the day 7 (P7) and day 75 (P75) after the last seizure. Whereas there were no significant changes in single seizure group and recurrent seizure group of P75, NR1 expression enhanced significantly in P7 rats of recurrent seizure group. Meanwhile, polypeptide levels of GABA-A-alpha 1 receptor subunit decreased significantly in both single and recurrent seizure-treated P7 and P75 rats. Our results suggest that recurrent or single prolonged seizures during the neonatal period may have long-term effects on the balance between excitatory NMDA system and inhibitory GABA system in hippocampus of rats.
为评估新生大鼠癫痫发作后惊厥阈值降低的病理生理机制，通过吸入三氟乙烷诱导新生大鼠出现单次或反复的长时间癫痫发作。在末次癫痫发作后的第7天（P7）和第75天（P75），采用蛋白质免疫印迹分析检测海马中N-甲基-D-天冬氨酸受体1（NR1）和γ-氨基丁酸Aα1受体（GABA-A-α1）免疫反应性的表达。虽然P75的单次癫痫发作组和反复癫痫发作组没有显著变化，但反复癫痫发作组的P7大鼠中NR1表达显著增强。同时，单次和反复癫痫发作处理的P7和P75大鼠中GABA-A-α1受体亚基的多肽水平均显著降低。我们的结果表明，新生期反复或单次长时间癫痫发作可能会对大鼠海马中兴奋性N-甲基-D-天冬氨酸（NMDA）系统和抑制性γ-氨基丁酸（GABA）系统之间的平衡产生长期影响。
