Gomez-Vargas Andrew, Rosenthal Kenneth L, McDermott Mark R, Hortelano Gonzalo
Department of Pathology and Molecular Medicine, McMaster University Health Sciences Centre, Hamilton, Ont., Canada L8N 3Z5.
Vaccine. 2004 Sep 28;22(29-30):3902-10. doi: 10.1016/j.vaccine.2004.04.010.
Protection against diseases is mediated by a sustained immune response. Here, we describe a new immunization strategy. Mice implanted with encapsulated C2C12 myoblasts secreting human factor IX (hFIX) elicited a strong humoral response against the transgene, as compared to mice immunized with complete Freund's adjuvant (FA). Mice also had increasing IgG2a antibody titer, indicating a switch to a Th1 profile immune response. Mice developed strong hFIX-specific cytotoxic T lymphocytes (CTL) that was detectable 213 days after implantation, demonstrating the sustained immunity elicited by encapsulated cells. Here, we propose continuous antigenic stimulation system (CASS) as a novel immunization strategy with potential application in the design of novel vaccines.
对疾病的保护是由持续的免疫反应介导的。在此，我们描述了一种新的免疫策略。与用完全弗氏佐剂（FA）免疫的小鼠相比，植入分泌人凝血因子IX（hFIX）的封装C2C12成肌细胞的小鼠引发了针对转基因的强烈体液反应。小鼠的IgG2a抗体滴度也不断增加，表明免疫反应转向了Th1型。小鼠产生了强大的hFIX特异性细胞毒性T淋巴细胞（CTL），在植入后213天仍可检测到，这证明了封装细胞引发的持续免疫。在此，我们提出连续抗原刺激系统（CASS）作为一种新型免疫策略，在新型疫苗设计中具有潜在应用价值。
