Kumar Pradeep, Paul Bholanath, Kumar Sirish, Ali Mohammad, Sexana Ashok K
Neurotoxicology Division, Indistrial Toxicology Research Centre, G.M. Marg, P.O. Box 80, Lucknow 226-001, India.
J Herb Pharmacother. 2004;4(2):33-45.
We attempt to derive a correlation between pro-inflammatory cytokines and mobility in arthritic mice and after treatment with Swertia chirayita plant extract. Tumor necrosis factor-á (TNF-á), interleukin-1alpha (IL-1alpha), interleukin-6 (IL-6) and interleukin-10 (IL-10) were measured by solid phase sandwich ELISA and the different parameters of mobility [distance traveled (DT), resting time (RT), ambulatory time (AT) and stereotypic time (ST)] was monitored and recorded by an Auto track system. Joint circumference of the right hind limb was monitored using a Vernier caliper. Our preliminary findings revealed that DT and RT correlated well with IL-1alpha, TNF-alpha and IL-10 levels in the joint homogenates of arthritic mice and after therapy with Swertia chirayita extracts. AT and ST showed poor correlation with the cytokines. Thus we demonstrate here the anti-inflammatory property of Swertia chirayita and its usage in the prevention of arthritis in the broader aspect in the management of associated cytokines.
我们试图推导促炎细胞因子与关节炎小鼠及獐牙菜植物提取物治疗后活动能力之间的相关性。通过固相夹心酶联免疫吸附测定法检测肿瘤坏死因子-α（TNF-α）、白细胞介素-1α（IL-1α）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10），并使用自动跟踪系统监测和记录活动能力的不同参数[行进距离（DT）、静息时间（RT）、行走时间（AT）和刻板行为时间（ST）]。使用游标卡尺监测右后肢的关节周长。我们的初步研究结果显示，在关节炎小鼠关节匀浆中以及獐牙菜提取物治疗后，DT和RT与IL-1α、TNF-α和IL-10水平具有良好的相关性。AT和ST与细胞因子的相关性较差。因此，我们在此证明了獐牙菜的抗炎特性及其在预防关节炎方面在更广泛的相关细胞因子管理中的用途。
