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[食品可追溯性的技术与可能性:家畜群体基因分型对食品安全的创新性贡献]

[Techniques and possibilities of traceability of food: genotyping of the domestic animal population as an innovative contribution to food safety].

作者信息

Brem G

机构信息

Veterinärmedizinische Universität Wien.

出版信息

Dtsch Tierarztl Wochenschr. 2004 Jul;111(7):273-6.

Abstract

Traceability of meat has become a very important aspect of quality assurance of food. DNA analyses could be used for identification and verification of farm animals and animal derived products. A prerequisite is the collection of qualified samples from entire populations of production animals or from regionally or specially characterised animal populations. The expenditure for conventional carrying out collection, preservation, cataloguing, and storage would be enormous. Therefore we have developed a simple, reliable, and inexpensive method for the collection using the ear tagging process and for preservation of samples at room temperature. A similar collection technology can also be used for sampling of carcasses, meat and meat products. Isolation of DNA from these tissue samples can be preformed using a new single step technology. For identifying individuals microsatellites and single nucleotide polymorphisms are analysed. Comparison of DNA fingerprints or SNP signatures allows to traceback samples collected from products to the animals they are coming from. If the system will be established on a nationwide basis the total costs would be less than 0.05 EUR per kilogram meat sold.

摘要

肉类可追溯性已成为食品质量保证的一个非常重要的方面。DNA分析可用于农场动物及动物源性产品的识别与验证。一个前提条件是从整个生产动物群体或具有区域或特殊特征的动物群体中采集合格样本。以传统方式进行样本采集、保存、编目和存储的费用将是巨大的。因此,我们开发了一种简单、可靠且廉价的方法,利用耳标过程进行样本采集,并在室温下保存样本。类似的采集技术也可用于屠体、肉类和肉制品的采样。可使用一种新的单步技术从这些组织样本中分离DNA。为识别个体,对微卫星和单核苷酸多态性进行分析。比较DNA指纹或SNP特征可追溯从产品采集的样本至其来源动物。如果该系统在全国范围内建立,每售出一公斤肉类的总成本将低于0.05欧元。

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