一种基于微珠的新型化学发光免疫分析法用于检测人促甲状腺激素
[A novel microbeads-based chemiluminescence immunoassay for detecting human thyrotropin].
作者信息
Wang Yong-ning, Li Rong-xiu, Li Cheng-wu, Gao Yun-hua, Guo Xiao-ying, Gu Zhong-ze
机构信息
Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing 100101, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Sep;20(5):629-31.
AIM
To establish a low cost and sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for detecting human thyrotropin.
METHODS
Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.
RESULTS
The sensitivity of this assay is 0.004 mIU/L. The intra-assay CV and the inter-assay CV of the assay were 7.45% and 10.45%, respectively. The recovery rate was 91.4% -102.4%. The examination result of the assay correlates well with that of ACS-180 system.
CONCLUSION
The CLELA for detecting TSH is low-cost, sensitive, specific and stable, and therefore may have a promising prospect in clinical application.
目的
建立一种用于检测人促甲状腺激素的低成本、高灵敏度的基于微珠的化学发光免疫分析方法（CLEIA）。
方法
血清中的促甲状腺激素由碱性磷酸酶标记的抗促甲状腺激素β亚基单克隆抗体和异硫氰酸荧光素标记的抗促甲状腺激素α亚基单克隆抗体捕获。然后用与抗异硫氰酸荧光素抗体偶联的免疫磁珠分离捕获的促甲状腺激素，并用金刚烷胺作为发光底物通过化学发光进行定量。
结果
该检测方法的灵敏度为0.004 mIU/L。该检测方法的批内变异系数和批间变异系数分别为7.45%和10.45%。回收率为91.4% - 102.4%。该检测方法的检测结果与ACS - 180系统的检测结果相关性良好。
结论
用于检测促甲状腺激素的CLEIA成本低、灵敏度高、特异性强且稳定，因此在临床应用中可能具有广阔的前景。
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