法国西南部从未去过肝炎流行地区的人群中的戊型肝炎

Hepatitis E in the south west of France in individuals who have never visited an endemic area.

作者信息

Mansuy Jean Michel, Peron Jean Marie, Abravanel Florence, Poirson Hélène, Dubois Martine, Miedouge Marcel, Vischi Françoise, Alric Laurent, Vinel Jean Pierre, Izopet Jacques

机构信息

Virology Laboratory, Purpan University Hospital, Place du Dr Baylac TSA 40031, 31059, Toulouse, France.

出版信息

J Med Virol. 2004 Nov;74(3):419-24. doi: 10.1002/jmv.20206.

DOI:10.1002/jmv.20206

PMID:15368508

Abstract

A total of 431 consecutive patients from the Midi Pyrenees area with acute hepatitis with unknown etiology in 2001-2002 were tested for the presence of immunoglobulin G-class (IgG) anti-hepatitis E virus (HEV) antibodies. Forty-six (10.7%) had anti-HEV IgG, and the results were questionable for a further 17 (3.9%). Real time PCR based on TaqMan detection was used to identify HEV genome fragments in the serum of patients with positive or questionable anti-HEV serology. HEV RNA was found in 25.4% of cases. All amplification products were sequenced and analyzed. Phylogenetic analysis revealed that all the strains were genotype 3. In conclusion, virological and epidemiological data indicate that genotype 3 viruses are circulating in the south west part of France (Midi-Pyrenees) in patients with acute hepatitis and who have not visited recently areas in which HEV is endemic.

摘要

2001年至2002年，对来自法国南部比利牛斯地区的431例病因不明的急性肝炎患者连续进行检测，以确定其是否存在免疫球蛋白G类（IgG）抗戊型肝炎病毒（HEV）抗体。其中46例（10.7%）抗HEV IgG呈阳性，另有17例（3.9%）结果存疑。采用基于TaqMan检测的实时PCR技术，对血清抗HEV血清学阳性或存疑患者的血清进行HEV基因组片段鉴定。结果发现，25.4%的病例中存在HEV RNA。对所有扩增产物进行测序和分析。系统发育分析表明，所有菌株均为3型。总之，病毒学和流行病学数据表明，3型病毒在法国西南部（南部比利牛斯）急性肝炎患者中传播，这些患者近期未去过戊型肝炎流行地区。