Tagarelli Antonio, Piro Anna, Tagarelli Giuseppe, Bastone Loredana, Paleari Renata, Mosca Andrea
Istituto di Scienze Neurologiche-CNR, Mangone (Cosenza), Italy.
Clin Biochem. 2004 Oct;37(10):863-6. doi: 10.1016/j.clinbiochem.2004.06.011.
To determine if measuring the ratio of glucose-6-phosphate dehydrogenase (G6PD) to pyruvate kinase (PK) is more reliable than only measuring G6PD activity to identify heterozygous G6PD- individuals with associated microcytic anemia in the Calabrian population, which shows high frequencies of both the thalassaemia (thal) trait and G6PD deficiency.
Measurement of G6PD and PK activities was carried out on 205 samples of whole blood from Calabrian subjects of both sexes (age range 10-50 years) using a double starter differential pH-metry technique.
The G6PD/PK ratio is able to differentiate G6PD- heterozygous individuals from the normal population. G6PD/PK values also allowed us to easily identify the G6PD- heterozygous subjects with microcytic anaemia. Student's t test shows that G6PD/PK ratio is more reliable in both sample groups, relative to G6PD activity in normal subjects.
G6PD/PK ratio is a reliable diagnostic parameter for mass screening for G6PD deficiency.
在卡拉布里亚人群中,地中海贫血(thal)特征和葡萄糖-6-磷酸脱氢酶(G6PD)缺乏症的发生率都很高,本研究旨在确定测量葡萄糖-6-磷酸脱氢酶(G6PD)与丙酮酸激酶(PK)的比值是否比仅测量G6PD活性更可靠,以识别患有相关小细胞贫血的杂合子G6PD缺乏个体。
采用双启动子差分pH测定技术,对205例来自卡拉布里亚地区10至50岁男女受试者的全血样本进行G6PD和PK活性测定。
G6PD/PK比值能够区分G6PD缺乏杂合子个体与正常人群。G6PD/PK值还使我们能够轻松识别患有小细胞贫血的G6PD缺乏杂合子受试者。学生t检验表明,相对于正常受试者的G6PD活性,G6PD/PK比值在两个样本组中都更可靠。
G6PD/PK比值是大规模筛查G6PD缺乏症的可靠诊断参数。