Amiwero C E, Olatunji P O
Department of Pathology, Specialist Hospital, Bauchi, Nigeria.
Afr J Med Med Sci. 2007 Jun;36(2):177-81.
Methaemoglobin reduction method is considered to be an old method of qualitative analysis of glucose-6-phosphate dehydrogenase with low specificity and sensitivity and as such, many are sceptical about the reliability of the outcome of the result of the methodology.The aims of this study were to determine the sensitivity and specificity of methaemoglobin reduction method and examine its suitability as a routine method for the screening of glucose-6-phosphate dehydrogenase in this environment. One hundred children were recruited into the study which was conducted over a period of one year. The children (males and females) were aged between 1 day and 15 years. G6PD screening by methaemoglobin reduction method was carried out on all the subjects, while G6PD qualitative assay using G6PD assay kits by Biolabo (France), was carried out in the first 15 subjects who were G6PD deficient by the qualitative screening and the first 15 subjects who were G6PD normal. This was to compare the qualitative with the quantitative assay results and thereby determine the sensitivity and specificity of the methaemoglobin reduction method. The sensitivity and specificity of methaemoglobin reduction test was found to be 93.3 percent each. The results of the qualitative methaemoglobin reduction test for the G6PD deficiency compared well with the quantitative enzymatic assay. With sensitivity and specificity each of 93.3%, the simple qualitative test is dependable and therefore suitable for screening for G6PD.
高铁血红蛋白还原法被认为是一种用于葡萄糖-6-磷酸脱氢酶定性分析的旧方法，其特异性和灵敏度较低，因此，许多人对该方法结果的可靠性持怀疑态度。本研究的目的是确定高铁血红蛋白还原法的灵敏度和特异性，并检验其作为在本环境中筛查葡萄糖-6-磷酸脱氢酶常规方法的适用性。一百名儿童被纳入本研究，研究持续了一年时间。这些儿童（男童和女童）年龄在1天至15岁之间。对所有受试者采用高铁血红蛋白还原法进行葡萄糖-6-磷酸脱氢酶筛查，而对定性筛查为葡萄糖-6-磷酸脱氢酶缺乏的前15名受试者以及葡萄糖-6-磷酸脱氢酶正常的前15名受试者，使用法国Biolabo公司的葡萄糖-6-磷酸脱氢酶检测试剂盒进行葡萄糖-6-磷酸脱氢酶定性检测。这是为了将定性检测结果与定量检测结果进行比较，从而确定高铁血红蛋白还原法的灵敏度和特异性。发现高铁血红蛋白还原试验的灵敏度和特异性均为93.3%。葡萄糖-6-磷酸脱氢酶缺乏的高铁血红蛋白还原定性试验结果与定量酶法检测结果吻合良好。由于灵敏度和特异性均为93.3%，这种简单的定性试验是可靠的，因此适用于葡萄糖-6-磷酸脱氢酶的筛查。
