通过针对katG和gyrA基因的双重实时荧光定量PCR检测结核分枝杆菌基因型组
Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes.
作者信息
Rindi Laura, Lari Nicoletta, Bonanni Daniela, Garzelli Carlo
机构信息
Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Via San Zeno, 35-39, Università di Pisa, I-56127 Pisa, Italy.
出版信息
J Microbiol Methods. 2004 Nov;59(2):283-7. doi: 10.1016/j.mimet.2004.06.012.
PMID:15369864
Abstract
A duplex real-time PCR assay was developed for the assignment of Mycobacterium tuberculosis isolates to the three genotypic groups based on the katG463/gyrA95 polymorphism. The assay was as sensitive and specific as nucleotide sequencing and proved also able to detect unambiguously the isolate genotype in clinical specimens.
摘要
基于katG463/gyrA95多态性，开发了一种双重实时PCR检测方法，用于将结核分枝杆菌分离株分为三个基因型组。该检测方法与核苷酸测序一样灵敏且特异，并且还证明能够明确检测临床标本中的分离株基因型。
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