用于检测北京基因型结核分枝杆菌 mutT4 和 mutT2 突变的双重实时 PCR 检测法。
A duplex real-time PCR assay for detection of mutT4 and mutT2 mutations in Mycobacterium tuberculosis of Beijing genotype.
机构信息
Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, I-56127 Pisa, Italy.
出版信息
J Microbiol Methods. 2010 May;81(2):203-4. doi: 10.1016/j.mimet.2010.03.004. Epub 2010 Mar 4.
PMID:20211667
Abstract
To determine the polymorphism of mutT genes of Mycobacterium tuberculosis of Beijing genotype, we developed a duplex real-time PCR assay based on hybridization probes for the Roche LightCycler instrument. The assay rapidly detects mutations at codons 48 and 58 of genes mutT4 and at mutT2, respectively.
摘要
为了确定北京基因型结核分枝杆菌 mutT 基因的多态性，我们开发了一种基于罗氏 LightCycler 仪器杂交探针的双重实时 PCR 检测方法。该检测方法能够快速检测 mutT4 基因的 48 位和 58 位密码子以及 mutT2 基因的突变。
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