反流性肾病患儿血清可溶性血管细胞黏附分子-1（VCAM-1）浓度

Serum soluble vascular cell adhesion molecule-1 (VCAM-1) concentrations in children with reflux nephropathy.

作者信息

Kaneyama Kazuhiro, Kobayashi Hiroyuki, Yamataka Atsuyuki, Lane Geoffrey J, Miyano Takeshi

机构信息

Department of Pediatric General & Urogenital Surgery, Juntendo University School of Medicine, 2-1-1 Hongo, 113-8421 Bunkyo-ku, Tokyo, Japan.

出版信息

Pediatr Surg Int. 2005 Jan;21(1):39-42. doi: 10.1007/s00383-004-1260-1.

DOI:10.1007/s00383-004-1260-1

PMID:15372288

Abstract

Systemic inflammatory disorders causing renal tissue damage do so by the adherence of polymorphonuclear leukocytes to endothelium, a process that is mediated by cell surface adhesion molecules. We determined the circulating levels of serum vascular cell adhesion molecule-1 (VCAM-1) in vesicoureteric reflux (VUR) patients and investigated the relationship between serum VCAM-1, grade of VUR, and secondary renal scarring. Serum levels of VCAM-1 were measured in 53 children aged between 3 months and 15 years with VUR (13 had grade III, 29 had grade IV, and 11 had grade V) and 25 controls using ELISA. Radionuclide scanning was used to assess renal scarring. Renal scarring was found in 29 of the 53 subjects. Serum VCAM-1 was significantly higher in subjects with high grades of VUR without renal scarring (grade IV: 715.9+/-121.0 ng/ml; grade V: 778.5+/-33.2ng/ml) compared with subjects with grade III VUR without renal scarring (609.8+/-64.3ng/ml, p<0.01). Serum VCAM-1 was also significantly higher in subjects with high grades of VUR with renal scarring (grade IV: 791.2+/-131.9ng/ml; grade V: 1171.8+/-235.6 ng/ml) compared with subjects with grade III VUR with renal scarring (687.3+/-163.4 ng/ml, p<0.001).

摘要

导致肾组织损伤的全身性炎症性疾病是通过多形核白细胞黏附于内皮细胞来实现的，这一过程由细胞表面黏附分子介导。我们测定了膀胱输尿管反流（VUR）患者血清血管细胞黏附分子-1（VCAM-1）的循环水平，并研究了血清VCAM-1、VUR分级与继发性肾瘢痕形成之间的关系。采用酶联免疫吸附测定法（ELISA）测定了53例年龄在3个月至15岁之间的VUR患儿（13例为III级，29例为IV级，11例为V级）和25例对照者的血清VCAM-1水平。使用放射性核素扫描评估肾瘢痕形成。53名受试者中有29人发现有肾瘢痕形成。与无肾瘢痕形成的III级VUR受试者（609.8±64.3 ng/ml）相比，无肾瘢痕形成的高等级VUR受试者（IV级：715.9±121.0 ng/ml；V级：778.5±33.2 ng/ml）血清VCAM-1显著更高（p<0.01）。与有肾瘢痕形成的III级VUR受试者（687.3±163.4 ng/ml）相比，有肾瘢痕形成的高等级VUR受试者（IV级：791.2±131.9 ng/ml；V级：1171.8±235.6 ng/ml）血清VCAM-1也显著更高（p<0.001）。