György E, Toricelli P, Socol G, Iliescu M, Mayer I, Mihailescu I N, Bigi A, Werckman J
National Institute for Lasers, Plasma and Radiation Physics, 77125 Bucharest-Magurele, Romania.
J Biomed Mater Res A. 2004 Nov 1;71(2):353-8. doi: 10.1002/jbm.a.30172.
Mn(2+)-doped carbonated hydroxyapatite (Mn-CHA) thin films were obtained by pulsed laser deposition on Ti substrates. The results of the performed complementary diagnostic techniques, X-ray diffraction, infrared spectroscopy, X-ray photoelectron spectroscopy, and energy dispersive X-ray spectroscopy investigations indicate that the films are crystalline with a Ca/P ratio of about 1.64-1.66. The optimum conditions, when nearly stoichiometric crystalline thin films were deposited, were found to be 10 Pa oxygen pressure, 400 degrees C substrate temperature, and postdeposition heat treatment in water vapors at the same substrate temperature. The films were seeded with L929 fibroblast and hFOB1.19 osteoblast cells and subjected to in vitro tests. Both fibroblast and osteoblast cells have a good adherence on the Mn-CHA film and on the Ti or polystyrene references. Proliferation and viability tests showed that osteoblast cells growth on Mn-CHA-coated Ti was enhanced as compared to uncoated pure Ti surfaces. Caspase-1 activity was not affected significantly by the material, showing that Mn-CHA does not induce apoptosis of cultured cells. These results demonstrate that Mn-CHA films on Ti should provoke a faster osteointegration of the coated implants as compared to pure Ti. (c) 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 353-358, 2004.
通过脉冲激光沉积法在钛基底上制备了掺锰碳酸羟基磷灰石（Mn-CHA）薄膜。所进行的X射线衍射、红外光谱、X射线光电子能谱和能量色散X射线光谱等互补诊断技术的研究结果表明，这些薄膜为晶体，钙磷比约为1.64 - 1.66。发现当沉积接近化学计量的晶体薄膜时，最佳条件为氧气压力10 Pa、基底温度400℃以及在相同基底温度下于水蒸气中进行沉积后热处理。将L929成纤维细胞和hFOB1.19成骨细胞接种到薄膜上并进行体外测试。成纤维细胞和成骨细胞在Mn-CHA薄膜以及钛或聚苯乙烯对照物上均具有良好的附着性。增殖和活力测试表明，与未涂层的纯钛表面相比，在Mn-CHA涂层钛上的成骨细胞生长得到增强。半胱天冬酶-1活性未受到材料的显著影响，表明Mn-CHA不会诱导培养细胞凋亡。这些结果表明，与纯钛相比，钛上的Mn-CHA薄膜应能促使涂层植入物更快地实现骨整合。（c）2004年威利期刊公司。《生物医学材料研究杂志》71A：353 - 358，2004年。
