高效液相色谱法测定人血浆中的雷公藤甲素和雷公藤内酯酮
Determination of triptolide and triptonide in human plasma by high-performance liquid chromatography.
作者信息
Wang Zhaohong, Wen Jiao, Xing Junbo, Guo Dean
机构信息
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road 38, Beijing 100083, People's Republic of China.
出版信息
Biomed Chromatogr. 2005 Jan;19(1):60-2. doi: 10.1002/bmc.416.
An isocratic high-performance liquid chromatographic method for determination of triptolide and triptonide in human plasma is described. Plasma samples were extracted with OasisHLB solid-phase extraction (SPE) cartridges. After pretreatment, they were separated on a SymmetryShieldRP(18) column with a mobile phase of acetonitrile-water (40:60,v/v) at 40 degrees C. The effluent was monitored at UV 217 nm. Linearity (0.010-1.0 mg/L) was good, and the lower limit of detection was 3 ng/mL for triptolide and 4.5 ng/mL for triptonide (S/N = 3). The relative standard deviations of intra- and inter-day assay were less than 15% and the recoveries were better than 80%. The developed method was applied to the determination of triptolide and triptonide concentration in a patient's plasma after taking the medicament containing Tripterygium wilfordii Hook. F.
描述了一种用于测定人血浆中雷公藤甲素和雷公藤乙素的等度高效液相色谱法。血浆样品用OasisHLB固相萃取（SPE）柱进行萃取。预处理后，在SymmetryShieldRP(18)柱上进行分离，流动相为乙腈-水（40:60，v/v），温度为40℃。流出物在217nm紫外波长下进行监测。线性范围（0.010 - 1.0mg/L）良好，雷公藤甲素的检测下限为3ng/mL，雷公藤乙素的检测下限为4.5ng/mL（信噪比 = 3）。日内和日间测定的相对标准偏差均小于15%，回收率优于80%。所建立的方法应用于测定服用含雷公藤药材的患者血浆中雷公藤甲素和雷公藤乙素的浓度。
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