Chen Ligang, Yu Aimin, Zhuang Xiaodong, Zhang Kui, Wang Xiupin, Ding Lan, Zhang Hanqi
College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
Talanta. 2007 Nov 15;74(1):146-52. doi: 10.1016/j.talanta.2007.05.043. Epub 2007 May 31.
The high-performance liquid chromatography (HPLC) coupled with on-line solid phase extraction (SPE) and ultraviolet (UV) detection was developed for determining andrographolide and dehydroandrographolide in rabbit plasma. Plasma samples (100 microL) were injected directly into a C18 SPE column and the biological matrix was washed out for 6 min using 15% aqueous methanol. By rotation of the switching valve, andrographolide and dehydroandrographolide were eluted in the back-flush mode and transferred to the analytical column by the chromatographic mobile phase consisted of methanol:acetonitrile (ACN):water (50:10:40; v/v). The UV detection was performed at 225 nm. The calibration curves showed excellent linear relationship (R> or =0.9993) over the concentration range of 0.05-5.0 microg mL(-1). The within- and between-day precisions (R.S.D.) of two analytes were in the range of 1.2-6.5% and the accuracies were between 92.0% and 102.1%. Their recoveries were all greater than 94%. The limits of detection were 0.019 microg mL(-1) for andrographolide and 0.022 microg mL(-1) for dehydroandrographolide. This method was successfully applied to the plasma concentration-time curve study after oral administration of Andrographis paniculata Nees extract in rabbit.
建立了高效液相色谱（HPLC）结合在线固相萃取（SPE）和紫外（UV）检测法，用于测定兔血浆中的穿心莲内酯和脱水穿心莲内酯。将血浆样品（100 μL）直接注入C18 SPE柱，用15%的甲醇水溶液冲洗生物基质6分钟。通过旋转切换阀，穿心莲内酯和脱水穿心莲内酯以反冲模式洗脱，并通过由甲醇：乙腈（ACN）：水（50:10:40；v/v）组成的色谱流动相转移至分析柱。在225 nm处进行UV检测。校准曲线在0.05 - 5.0 μg mL-1的浓度范围内显示出良好的线性关系（R≥0.9993）。两种分析物的日内和日间精密度（R.S.D.）在1.2 - 6.5%范围内，准确度在92.0%至102.1%之间。它们的回收率均大于94%。穿心莲内酯的检测限为0.019 μg mL-1，脱水穿心莲内酯的检测限为0.022 μg mL-1。该方法成功应用于兔口服穿心莲提取物后的血浆浓度-时间曲线研究。
Zotero 插件