Serban Simona, Danet Andrei F, El Murr Nabil
LAIEM (UMR-CNRS 6006), FCMN (FR-CNRS 2465), Faculté des Sciences et des Techniques, Université de Nantes, 2 rue de la Houssinière, B.P. 92208, 44322 Nantes 03, France.
J Agric Food Chem. 2004 Sep 8;52(18):5588-92. doi: 10.1021/jf0494229.
A rapid and sensitive automated method for glucose monitoring that might be employed during wine fermentation and processing was developed. A flow injection (FI) system coupled with an automated dilutor and the "redox-versatile" modified electrode were used to directly measure glucose in wine. To avoid interferences during wine analysis, different formulations of enzymatically modified carbon paste electrodes (CPE) were used and evaluated in oxidation and reduction mode. The best selectivity and sensitivity for glucose monitoring in real samples was obtained in cathodic mode at a fixed potential of 0 V versus Ag/AgCl using a CPE modified with glucose oxidase, horseradish peroxidase, and ferrocene as redox mediator. A total linear range of 0.02-50 g/L glucose was covered using this automated system and allowed the measurement of glucose in dry, medium, and sweet white or red wines without any sample pretreatment. The results showed a good correlation with the standard method, and the proposed method is very rapid, simple, and reliable and does not need skilled operators.
开发了一种可用于葡萄酒发酵和加工过程中葡萄糖监测的快速灵敏的自动化方法。采用流动注射（FI）系统与自动稀释器以及“氧化还原通用”修饰电极联用，直接测定葡萄酒中的葡萄糖。为避免葡萄酒分析过程中的干扰，使用了不同配方的酶修饰碳糊电极（CPE），并在氧化和还原模式下进行了评估。使用以葡萄糖氧化酶、辣根过氧化物酶和二茂铁作为氧化还原介质修饰的CPE，在相对于Ag/AgCl为0 V的固定电位下，于阴极模式获得了实际样品中葡萄糖监测的最佳选择性和灵敏度。使用该自动化系统，葡萄糖的总线性范围为0.02 - 50 g/L，无需任何样品预处理即可测定干型、半干型和甜型白葡萄酒或红葡萄酒中的葡萄糖。结果与标准方法具有良好的相关性，所提出的方法非常快速、简单且可靠，不需要熟练的操作人员。
