Zehentner Barbara K, Persing David H, Deme Amadou, Toure Papa, Hawes Stephen E, Brooks Lisa, Feng Qinghua, Hayes Dawn C, Critichlow Cathy W, Houghton Raymond L, Kiviat Nancy B
Corixa Corporation, Seattle, WA 98101, USA.
Clin Chem. 2004 Nov;50(11):2069-76. doi: 10.1373/clinchem.2004.038687. Epub 2004 Sep 16.
The aim of this study was to examine the potential usefulness of a mammaglobin multigene reverse transcription-PCR (RT-PCR) assay and a mammaglobin sandwich ELISA as diagnostic tools in breast cancer.
We studied peripheral blood samples from 147 untreated Senegalese women with biopsy-confirmed breast cancer and gathered patient information regarding demographic, and clinical staging of disease. The samples were tested for mammaglobin and three breast cancer-associated gene transcripts by a multigene real-time RT-PCR assay and for serum mammaglobin protein by a sandwich ELISA assay.
In 77% of the breast cancer blood samples, a positive signal was obtained in the multigene RT-PCR assay detecting mammaglobin and three complementary transcribed genes. Fifty samples from healthy female donors tested negative. Significant correlations were found between mammaglobin protein in serum, presence of mammaglobin mRNA-expressing cells in blood, stage of disease, and tumor size. Circulating mammaglobin protein was detected in 68% of the breast cancer sera, and was increased in 38% in comparison with a mixed control population. The RT-PCR assay and the ELISA for mammaglobin produced a combined sensitivity of 84% and specificity of 97%.
The ELISA and RT-PCR for mammaglobin and mammaglobin-producing cells could be valuable tools for diagnosis and prognosis of breast cancer.
本研究的目的是检测乳腺珠蛋白多基因逆转录聚合酶链反应（RT-PCR）检测法和乳腺珠蛋白夹心酶联免疫吸附测定法（ELISA）作为乳腺癌诊断工具的潜在效用。
我们研究了147名未经治疗、经活检确诊为乳腺癌的塞内加尔女性的外周血样本，并收集了患者的人口统计学信息和疾病临床分期信息。通过多基因实时RT-PCR检测法检测样本中的乳腺珠蛋白和三种乳腺癌相关基因转录本，并通过夹心ELISA检测法检测血清中的乳腺珠蛋白蛋白。
在77%的乳腺癌血液样本中，多基因RT-PCR检测法在检测乳腺珠蛋白和三种互补转录基因时获得了阳性信号。来自健康女性捐赠者的50个样本检测为阴性。血清中的乳腺珠蛋白蛋白、血液中表达乳腺珠蛋白mRNA的细胞的存在、疾病分期和肿瘤大小之间存在显著相关性。在68%的乳腺癌血清中检测到循环乳腺珠蛋白蛋白，与混合对照人群相比增加了38%。乳腺珠蛋白RT-PCR检测法和ELISA的联合敏感性为84%，特异性为97%。
乳腺珠蛋白和产生乳腺珠蛋白的细胞的ELISA和RT-PCR可能是乳腺癌诊断和预后的有价值工具。
